Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [RNA-Seq]

ABSTRACT: Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [RNA-Seq]

PROVIDER: PRJNA947230 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23932234.fastq.gz	Fastqsanger.gz
	SRR23932235.fastq.gz	Fastqsanger.gz
	SRR23932236.fastq.gz	Fastqsanger.gz
	SRR23932237.fastq.gz	Fastqsanger.gz
	SRR23932238.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function

Project description:Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function

| PRJNA947223 | ENA

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [ChIP-Seq]

Project description:Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [ChIP-Seq]

| PRJNA947229 | ENA

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [ATAC-Seq]

Project description:Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [ATAC-Seq]

| PRJNA947231 | ENA

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [CUT&RUN]

Project description:Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [CUT&RUN]

| PRJNA947228 | ENA

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [ATAC-Seq]

Project description:While chromatin presents a barrier to the binding of many transcription factors, pioneer factors access nucleosomal targets and promote chromatin opening. Despite binding to target motifs in closed chromatin, many pioneer factors display cell-type specific binding and activity. The mechanisms governing pioneer-factor occupancy and the relationship between chromatin occupancy and opening remain unclear. We studied three Drosophila transcription factors with distinct DNA-binding domains and biological functions: Zelda, Grainy head, and Twist. We demonstrated that the level of chromatin occupancy is a key determinant of activity. Multiple factors regulate occupancy, including motif content, local chromatin, and protein concentration. Regions outside the DNA-binding domain are required for binding and chromatin opening. Our results show that pioneering activity is not a binary feature intrinsic to a protein but occurs on a spectrum and is regulated by a variety of protein-intrinsic and cell-type-specific features.

2023-05-02 | GSE227880 | GEO

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [RNA-Seq]

2023-05-02 | GSE227883 | GEO

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [CUT&RUN]

2023-05-02 | GSE227881 | GEO

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function [ChIP-Seq]

2023-05-02 | GSE227882 | GEO

Protein-intrinsic properties and context-dependent effects regulate pioneer factor binding and function.

Project description:Chromatin is a barrier to the binding of many transcription factors. By contrast, pioneer factors access nucleosomal targets and promote chromatin opening. Despite binding to target motifs in closed chromatin, many pioneer factors display cell-type-specific binding and activity. The mechanisms governing pioneer factor occupancy and the relationship between chromatin occupancy and opening remain unclear. We studied three Drosophila transcription factors with distinct DNA-binding domains and biological functions: Zelda, Grainy head and Twist. We demonstrated that the level of chromatin occupancy is a key determinant of pioneering activity. Multiple factors regulate occupancy, including motif content, local chromatin and protein concentration. Regions outside the DNA-binding domain are required for binding and chromatin opening. Our results show that pioneering activity is not a binary feature intrinsic to a protein but occurs on a spectrum and is regulated by a variety of protein-intrinsic and cell-type-specific features.

| S-EPMC11261375 | biostudies-literature

Protein-intrinsic properties and context-dependent effects regulate pioneer-factor binding and function.

Project description:Chromatin is a barrier to the binding of many transcription factors. By contrast, pioneer factors access nucleosomal targets and promote chromatin opening. Despite binding to target motifs in closed chromatin, many pioneer factors display cell-type specific binding and activity. The mechanisms governing pioneer-factor occupancy and the relationship between chromatin occupancy and opening remain unclear. We studied three Drosophila transcription factors with distinct DNA-binding domains and biological functions: Zelda, Grainy head, and Twist. We demonstrated that the level of chromatin occupancy is a key determinant of pioneering activity. Multiple factors regulate occupancy, including motif content, local chromatin, and protein concentration. Regions outside the DNA-binding domain are required for binding and chromatin opening. Our results show that pioneering activity is not a binary feature intrinsic to a protein but occurs on a spectrum and is regulated by a variety of protein-intrinsic and cell-type-specific features.

| S-EPMC10103944 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data