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Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [SOX9 MO ATAC-seq]

ABSTRACT: Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [SOX9 MO ATAC-seq]

PROVIDER: PRJNA949373 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23985093_1.fastq.gz	Fastqsanger.gz
	SRR23985093_2.fastq.gz	Fastqsanger.gz
	SRR23985094_1.fastq.gz	Fastqsanger.gz
	SRR23985094_2.fastq.gz	Fastqsanger.gz
	SRR23985095_1.fastq.gz	Fastqsanger.gz

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Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [SOX9 MO ATAC-seq]

Project description:Embryonic cells engage in diverse types of metabolism to execute specialized tasks in the developing embryo. Recent studies have demonstrated that metabolic reprogramming can also drive changes in cell identity and behavior by affecting the expression of developmental genes. However, the connection between cellular metabolism and differential gene expression is still not well understood. Here we report found that histone lactylation, an epigenetic mark derived from glycolysis-derived lactate, couples the metabolic state of embryonic cells with gene expression and the activation of gene regulatory networks. Embryonic tissues with high glycolytic flux, like the neural crest and the pre-somitic mesoderm, display high levels of lactylation. The lactylation mark is dynamically deposited in the loci of neural crest genes as these cells transition to a state of enhanced glycolysis. This process promotes accessibility of active enhancers and is necessary for proper deployment of the neural crest gene regulatory network. When we reduced the deposition of the mark by targeting LDHA and LDHB, lactylated genes were downregulated, and neural crest migration was impeded. Lactylation of neural crest enhancers is controlled by transcription factors SOX9 and YAP/TEAD, which are necessary and sufficient for the deposition of the mark. These findings define an epigenetic mechanism that integrates cellular metabolism with the gene regulatory networks that orchestrate embryonic development.

2023-11-22 | GSE228341 | GEO

Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [CUT&RUN control and SOX9/TEA1-VPR over-expressing]

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Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [scATAC-seq]

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Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [NCC ATAC-seq]

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Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [NCC CUT&RUN]

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Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [R-GNE-140 ATAC-seq]

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Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks

Project description:Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks

| PRJNA949368 | ENA

Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [CUT&RUN control and SOX9/TEA1-VPR over-expressing]

Project description:Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [CUT&RUN control and SOX9/TEA1-VPR over-expressing]

| PRJNA1007594 | ENA

Histone lactylation couples cellular metabolism with developmental gene regulatory networks.

Project description:Embryonic cells exhibit diverse metabolic states. Recent studies have demonstrated that metabolic reprogramming drives changes in cell identity by affecting gene expression. However, the connection between cellular metabolism and gene expression remains poorly understood. Here we report that glycolysis-regulated histone lactylation couples the metabolic state of embryonic cells with chromatin organization and gene regulatory network (GRN) activation. We found that lactylation marks genomic regions of glycolytic embryonic tissues, like the neural crest (NC) and pre-somitic mesoderm. Histone lactylation occurs in the loci of NC genes as these cells upregulate glycolysis. This process promotes the accessibility of active enhancers and the deployment of the NC GRN. Reducing the deposition of the mark by targeting LDHA/B leads to the downregulation of NC genes and the impairment of cell migration. The deposition of lactyl-CoA on histones at NC enhancers is supported by a mechanism that involves transcription factors SOX9 and YAP/TEAD. These findings define an epigenetic mechanism that integrates cellular metabolism with the GRNs that orchestrate embryonic development.

| S-EPMC10762033 | biostudies-literature

Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [scATAC-seq]

Project description:Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [scATAC-seq]

| PRJNA949371 | ENA