Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: mRNA sequencing of mock or BmNPV infected BmN cells

PROVIDER: PRJNA949399 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23985227_1.fastq.gz	Fastqsanger.gz
	SRR23985227_2.fastq.gz	Fastqsanger.gz
	SRR23985228_1.fastq.gz	Fastqsanger.gz
	SRR23985228_2.fastq.gz	Fastqsanger.gz
	SRR23985229_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Comprehensive Profiling of Lysine Acetylome in Baculovirus Infected Silkworm (Bombyx mori) Cells

Project description:Bombyx mori is one of the key lepidopteran model species, and is economically important for silk production and proteinaceous drug expression. Baculovirus and insect host are important natural biological models for studying host-pathogen interactions. The impact of Bombyx mori nucleopolyhedrovirus (BmNPV) infection on the proteome and acetylome of Bombyx mori ovarian (BmN) cells were explored to facilitate a better understanding of infection-driven interactions between BmNPV and host in vitro. The proteome and acetylome were profiled through 6-plex Tandem mass tag (TMT) labelling-based quantitative proteomics. Totally, 4,194 host proteins were quantified, of which 33 were up-regulated and 47 were down-regulated in BmN cells at 36 h post-infection. Based on the proteome, quantifiable differential Kac proteins were identified and functionally annotated to gene expression regulation, energy metabolism, substance synthesis and metabolism after BmNPV infection. Altogether, 644 Kac sites in 431 host proteins and 39 Kac sites in 22 viral proteins were identified and quantified in infected BmN cells. Our study demonstrated that BmNPV infection globally impacts the proteome and acetylome of BmN cells. The viral proteins are also acetylated by the host acetyltransferase. Protein acetylation is essential for cellular self-regulation and response to virus infection. This study provides new insights for understanding the host-virus interaction mechanisms, and the role of acetylation in BmN cellular response to viral infection.

2017-11-27 | PXD008054 | Pride

Project description:Ribo-seq analysis of BmNPV infected BmN cells

| PRJNA1296756 | ENA

Project description:RNA-seq of BmNPV infected BmN cells 60 phi

| PRJNA1297027 | ENA

Project description:Small RNA sequencing of WT or p35KO BmNPV infected BmN cells

| PRJNA792944 | ENA

Bombyx mori nucleopolyhedrovirus strain:T3

Project description:Transcriptome analysis of BmNPV-infected BmN-4 cells with Bm8 mutants

| PRJDB13017 | ENA

Bombyx mori

Project description:Transcriptome and epigenomic analyses of BmN-4 cells infected with BmNPV

| PRJDB6462 | ENA

Quantitative proteomics of Bombyx mori after BmNPV challenge

Project description:Employed BmN cell after 10 MOI (multiplicity of infection) BmNPV 36 hpi (hour post-infection) to compare the proteomics by LC-MS/MS

2018-05-22 | PXD008856 | Pride

Project description:BmNPV recombinant baculovirus infects BmN

| PRJNA1262002 | ENA

Functional analysis of BmNPV cis-interacting sites

Project description:RNA-seq of BmNPV cells infected by indicated mutant BmNPV to determine the impact on viral gene expression of the cis-interacting sites on BmNPV by knocking out them via RED system

2024-10-01 | GSE278147 | GEO

Precocious metamorphosis of silkworm by BmNPV infection in the latter half of the fifth instar larvae

Project description:The silkworm, Bombyx mori, is a complete metamorphosis insect and an economically important for silk production, the model to study insect physiology and biochemistry. Bombyx mori nucleopolyhedrovirus (BmNPV) is a principal pathogen of the silkworm and its host range is restricted to silkworm larvae, requiring interaction with silkworm larvae to accomplish virus replication. Prothoracic glands (PGs) are a model for synthetic ecdysone with regulating insect growth and development. In this study, day-4 fifth instar silkworm larvae were infected by BmNPV, the wandering silkworms appeared in the infected groups were 12 hours earlier than that in the control groups, and the ecdysone titer in infected larvae was significantly higher than that of the control larvae. Then, we used RNA sequencing (RNA-seq) to analyze silkworm PGs 48 h after BmNPV infection. The classifications of the 15 differential expression genes (DEGs) were mainly involved in the metabolic processes and pathways. The RT-qPCR results of the DEGs in the PGs of BmNPV-infected at 24, 48, and 72 h were generally consistent with the transcriptome data. The transcripts of BmTrypsin-1 and BmACSS3 were significantly increased from 24 to 72 h after BmNPV infection that they may be involved in the maturation process in the latter half of silkworm fifth instar larvae. These findings will help to address the interactions between BmNPV infection and host developmental response.

2021-03-02 | GSE167875 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data