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ABSTRACT:
PROVIDER: PRJNA949416 | ENA |
REPOSITORIES: ENA
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Project description:DNA methylation is an important epigenetic modification. DNA methylation phenomenon exists widely in bacteria, plants and animals and is involved in a variety of biological processes.The wild Lactobacillus casei Zhang and its mutant Lactobacillus casei Zhang Δpglx were used as the research subjects.Proteomics was used to explore the effects of DNA methylation on various aspects of Lactobacillus casei.
2023-03-22 | PXD026826 | Pride
Project description:Objective: This study combines transcriptomics and network pharmacology to explore the mechanism of action of Jia Wei Xing Bi Gel nasal drops in treating allergic rhinitis (AR) in children. Methods: A total of 56 AR children were randomly divided into Jia Wei Xing Bi Gel nasal drops(JWXBGND) group and mometasone furoate nasal spray (MFGS) group, with 28 cases in each group, and a healthy control group of 20 cases. After 4 weeks of treatment, efficacy was evaluated, and RNA-seq, database mining, and bioinformatics analysis methods were employed. Results: In terms of clinical efficacy, the TCM group showed better improvement in nasal itching, ocular symptoms, non-nasal ocular symptoms, and behavioral issues compared to the western medicine group, with a more significant decrease in TCM syndrome scores; transcriptomic analysis revealed 2776 differentially expressed genes when comparing AR children with healthy children, and 441 differential genes were identified between JWXBGND group and MFGS group; combined analysis of transcriptomics and network pharmacology identified 195 active components and 493 targets of action for Jiawei Xingbi Gel, with core components including ferulic acid, fexofenadine, and curcumin A, and core targets involving TNF, IL-6, IL-1β, AKT1, ALB, SRC, etc.; KEGG pathway analysis mainly enriched in caffeine metabolism, linoleic acid metabolism, Th17 cell differentiation, HIF-1 signaling pathway, etc.; molecular docking results indicated that key active components of Jia Wei Xing Bi Gel nasal drops bind stably to targets such as ALB, AKT1, SRC, TP53, etc. Conclusion: Jia Wei Xing Bi Gel nasal drops can effectively alleviate AR symptoms, and its mechanism may be related to the regulation of inflammatory signaling pathways through multiple components and multiple targets
2026-03-07 | GSE322669 | GEO
Project description:Wei et al. performed microarray analysis to explore the metabolism change in diabetes and RYGB surgery treated rats.
2023-08-01 | GSE156908 | GEO
Project description:The survival of probiotics could be strongly enhanced by delivery vehicles, but the mechanism was unknown. In this study, whole genome microarray technology was used to detect the gene expression profiles of probiotic Lactobacillus casei Zhang in the absence and presence of fermented milk in simulated gastrointestinal tract. The results showed the gene expression profiles were significantly different under these two different conditions. The change of the gene expression profile may be helpful to comprehend the role of delivery vehicles in enhancing the survival of probiotics. Twelve samples of bacterial cells in the absence and presence of fermented milk were collected at 3 h in simulated gastric juice, 4 h and 8 h in intestinal juice, 2 biological replicates were obtained in each time point. The average of the normalized expression values of the 2 biological replicates for each probe was regarded as the expression value of a predicted gene. To identify the expression pattern of each gene across different treatments, n-fold change ratios were calculated for every gene in pure L. casei Zhang versus L. casei Zhang in fermented milk.
2011-12-31 | E-GEOD-23685 | biostudies-arrayexpress