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Effect of Arid1a deletion on chromatin accessibility in antiviral effector CD8+ T cells. [Cut & Run]

ABSTRACT: Effect of Arid1a deletion on chromatin accessibility in antiviral effector CD8+ T cells. [Cut & Run]

PROVIDER: PRJNA949603 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23994251_1.fastq.gz	Fastqsanger.gz
	SRR23994251_2.fastq.gz	Fastqsanger.gz
	SRR23994252_1.fastq.gz	Fastqsanger.gz
	SRR23994252_2.fastq.gz	Fastqsanger.gz
	SRR23994253_1.fastq.gz	Fastqsanger.gz

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Effect of Arid1a deletion on chromatin accessibility in antiviral effector CD8+ T cells. [Cut & Run]

Project description:To investigate the role of ARID1A in the regulation of transcription factor binding and histone modifications in effector CD8+ T cell responses, we crossed GzmB-Cre P14 Thy1.1 mice with Arid1a-fl/fl mice to generate Arid1a KO mice. We then performed CUT&RUN against ARID1A, BATF, ETS, Tbet, H3K27ac, H3K27me3 in WT, Arid1a KO, or Tbx21 (Tbet) KO cells in vivo at d0, 48h in vitro activated, d5, and d8 post-LCMV Armstrong infection.

2023-06-13 | GSE228380 | GEO

Effect of Arid1a deletion in antiviral effector CD8+ T cells. [RNA-seq]

Project description:To investigate the roll of ARID1A in the regulation of effector CD8+ T cell responses, we crossed GzmB-Cre P14 Thy1.1 mice with Arid1a-fl/fl mice to generate Arid1a Het and KO mice. We then performed gene expression profiling analysis of WT, Het, and KO cells in vivo at d3, d5, and d8 post-LCMV Armstrong infection.

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Effect of Arid1a deletion in antiviral effector CD8+ T cells. [RNA-seq]

Project description:Effect of Arid1a deletion in antiviral effector CD8+ T cells. [RNA-seq]

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Effect of Arid1a deletion on chromatin accessibility in antiviral effector CD8+ T cells. [ATAC-Seq]

Project description:To investigate the role of ARID1A in the regulation of effector CD8+ T cell responses, we crossed GzmB-Cre P14 Thy1.1 mice with Arid1a-fl/fl mice to generate Arid1a Het and KO mice. We then performed chromatin accessibility profiling analysis by ATAC-seq in WT, Het, and KO cells in vivo at d0, 48h in vitro activated, d3, d5, and d8 post-LCMV Armstrong infection.

2023-06-13 | GSE228171 | GEO

Effect of Arid1a deletion on chromatin accessibility in antiviral effector CD8+ T cells. [ATAC-Seq]

Project description:Effect of Arid1a deletion on chromatin accessibility in antiviral effector CD8+ T cells. [ATAC-Seq]

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Chromatin remodeler ARID1A binds IRF3 to selectively induce antiviral interferon production in macrophages

Project description:Transcription factor IRF3 is critical for the induction of antiviral type I interferon (IFN-I). The epigenetic regulation of IFN-I production in antiviral innate immunity need to be further identified. Here, we report that epigenetic remodeler ARID1A, a critical component of the mSWI/SNF complex, could bind IRF3 and then was recruited to the Ifn-I promoter by IRF3, thus selectively promoting IFN-I but not TNF-α, IL-6 production in macrophages upon viral infection. Myeloid cell-specific deficiency of Arid1a rendered mice more susceptible to viral infection, accompanied with less IFN-I production. Mechanistically, ARID1A facilitates chromatin accessibility of IRF3 at the Ifn-I promoter by interacting with histone methyltransferase NSD2, which methylates H3K4 and H3K36 of promoter region. Our findings demonstrate the new roles of ARID1A and NSD2 in innate immunity, providing insight to the crosstalks of chromatin remodeling, histone modification and transcription factor in the epigenetic regulation of antiviral innate immunity.

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ARID1A governs silencing of sex-linked transcription during male meiosis in the mouse [CUT&RUN]

Project description:We present evidence implicating the BAF (BRG1/BRM Associated Factor) chromatin remodeler in meiotic sex chromosome inactivation (MSCI). By immunofluorescence (IF), the putative BAF DNA binding subunit, ARID1A (AT-rich Interaction Domain 1a), appeared enriched on the male sex chromosomes during diplonema of meiosis I. The germ cell-specific depletion of ARID1A resulted in a pachynema arrest and failure to repress sex-linked genes indicating a defective MSCI. Consistent with this defect, mutant sex chromosomes displayed an abnormal presence of elongating RNA polymerase II coupled with an overall increase in chromatin accessibility detectable by ATAC-seq. By investigating potential mechanisms underlying these anomalies, we identified a role for ARID1A in promoting the preferential enrichment of the histone variant, H3.3, on the sex chromosomes, a known hallmark of MSCI. Without ARID1A, the sex chromosomes appeared depleted of H3.3 at levels resembling autosomes. Higher resolution analyses by CUT&RUN revealed dramatic shifts in sex-linked H3.3 associations from discrete intergenic sites and broader gene-body domains to promoters in response to the loss of ARID1A. Several sex-linked sites displayed ectopic H3.3 occupancy that does not co-localize with DMC1 (DNA Meiotic Recombinase 1). This observation suggests a requirement for ARID1A in DMC1 localization to the asynapsed sex chromatids. We conclude that ARID1A-directed H3.3 localization influences sex chromosome gene regulation and DNA repair during meiosis I.

2024-02-19 | GSE225609 | GEO

Co-existing TP53 and ARID1A mutations promote aggressive endometrial tumorigenesis [CUT&RUN]

Project description:Co-existing TP53 and ARID1A mutations promote aggressive endometrial tumorigenesis [CUT&RUN]

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Arid1a deletion in collaboration with Pten deletion leads to ovarian tumorigenesis in mouse

Project description:Mice developed ovarian tumors after Arid1a and Pten double knockout. Gene expression profiles of 5 such ovarian tumors were compared with those of 4 normal ovaries. The differentially expressed genes were then used to investigate the similarity between the mouse ovarian tumors and major subtypes of human ovarian cancers. Total RNA obtained from the mouse ovarian tumors developing after Arid1a and Pten knockout compared to normal mouse ovaries.

2014-04-05 | E-GEOD-56531 | biostudies-arrayexpress

Innate triggering and antiviral effector functions of activin A

Project description:Innate triggering and antiviral effector functions of activin A

| PRJNA786577 | ENA