Project description:Deep sequencing of mRNA from Spalax galili and Rattus norvegicus Total RNAs were isolated from the brain of female BMRs under various oxygen concentrations (3%, 6%, 10%, 14%, and 21%) for 6 hours. We also got RNAs from the brain of rats under 6%, 10%, 14%, and 21% oxygen conditions.
Project description:The aim of this project is to use an integrated approach involving TMT labeling and LC-MS/MS to quantify the dynamic changes of the whole proteome of chondrocytes of Rattus norvegicus.
Project description:A microarray analysis (transcriptome analysis) was conducted to examine estrogen-mediated gene expression in the arcuate nucleus (ARC) and paraventricular nucleus (PVN) of the hypothalamus, as well as the nucleus of the solitary tract (NTS) in female rats (Rattus norvegicus). All procedures were approved by the Oklahoma State University - Center for Health Sciences IACUC (protocol #2021-1278). Rats underwent ovariectomy, recovered, and were then treated with either 17β-estradiol (EB) or an oil vehicle (Oil). Following euthanasia, mRNA was extracted and submitted to Thermo Fisher Scientific for microarray analysis using the Clariom S Rat Pico Assay.
