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RNA polymerase II pausing is essential for appropriate gene expression and meiosis during spermatogenesis

ABSTRACT: RNA polymerase II pausing is essential for appropriate gene expression and meiosis during spermatogenesis

PROVIDER: PRJNA951360 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24042816.fastq.gz	Fastqsanger.gz
	SRR24042817.fastq.gz	Fastqsanger.gz
	SRR24042818.fastq.gz	Fastqsanger.gz
	SRR24042819.fastq.gz	Fastqsanger.gz
	SRR24042820.fastq.gz	Fastqsanger.gz

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RNA polymerase II pausing is essential for appropriate gene expression and meiosis during spermatogenesis

Project description:Male germ cell development requires precise regulation of gene activity, in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use both steady-state and nascent RNA sequencing strategies to comprehensively characterize gene expression across male germ cell populations, to dissect the mechanisms of gene control and provide new insights towards therapy. We discover a requirement for pausing of RNA Polymerase II (Pol II) at the earliest stages of sperm differentiation to establish the landscape of gene activity across development. Accordingly, genetic knockout of the Pol II pause-inducing factor NELF in immature germ cells impairs differentiation to mature spermatids. Moreover, our work reveals unanticipated roles for Pol II pausing in the regulation of meiosis during spermatogenesis, with the presence of paused Pol II influencing double strand break formation by Spo11, and disruption of Spo11 expression in germ cells lacking NELF.

2023-12-18 | GSE228722 | GEO

RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis

Project description:RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis

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RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis [RNA-Seq]

Project description:Male germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use steady-state, nascent and single-cell RNA sequencing strategies to comprehensively characterize gene expression across male germ cell populations, to dissect the mechanisms of gene control and provide new insights towards therapy. We discover a requirement for pausing of RNA Polymerase II (Pol II) at the earliest stages of sperm differentiation to establish the landscape of gene activity across development. Accordingly, genetic knockout of the Pol II pause-inducing factor NELF in immature germ cells blocks differentiation to spermatids. Further, we uncover unanticipated roles for Pol II pausing in the regulation of meiosis during spermatogenesis, with the presence of paused Pol II associated with double strand break (DSB) formation, and disruption of meiotic gene expression and DSB repair in germ cells lacking NELF.

2023-12-18 | GSE228453 | GEO

RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis[PRO-Seq]

2023-12-18 | GSE228452 | GEO

RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis[PRO-Seq]

Project description:RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis[PRO-Seq]

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RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis [RNA-Seq]

Project description:RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis [RNA-Seq]

| PRJNA949951 | ENA

RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis.

Project description:Male germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use steady-state, nascent and single-cell RNA sequencing strategies to comprehensively characterize gene expression across male germ cell populations, to dissect the mechanisms of gene control and provide new insights towards therapy. We discover a requirement for pausing of RNA Polymerase II (Pol II) at the earliest stages of sperm differentiation to establish the landscape of gene activity across development. Accordingly, genetic knockout of the Pol II pause-inducing factor NELF in immature germ cells blocks differentiation to mature spermatids. Further, we uncover unanticipated roles for Pol II pausing in the regulation of meiosis during spermatogenesis, with the presence of paused Pol II associated with double strand break formation by SPO11, and disruption of SPO11 expression in germ cells lacking NELF.

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RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis.

Project description:Male germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use steady-state, nascent and single-cell RNA sequencing strategies to comprehensively characterize gene expression across male germ cell populations, to dissect the mechanisms of gene control and provide new insights towards therapy. We discover a requirement for pausing of RNA Polymerase II (Pol II) at the earliest stages of sperm differentiation to establish the landscape of gene activity across development. Accordingly, genetic knockout of the Pol II pause-inducing factor NELF in immature germ cells blocks differentiation to spermatids. Further, we uncover unanticipated roles for Pol II pausing in the regulation of meiosis during spermatogenesis, with the presence of paused Pol II associated with double-strand break (DSB) formation, and disruption of meiotic gene expression and DSB repair in germ cells lacking NELF.

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DIS3 is essential for male germ cell meiosis and spermatogenesis in mice

Project description:Male germ cell meiosis is essential for generating haploid spermatozoa in mice. Here, we investigate the essential role of DIS3 in male germ cell meiosis in mice. Conditional inactivation of DIS3 in spermatocytes with Stra8-cre transgenic mice have severely impaired meiotic progression, which results in defective meiosis and spermatogenesis. RNA-seq analysis reveals that Dis3 deficiency causes significant dysregulation of the expression of transcripts in mutant testes. Meiosis-associated genes are significantly decreased in the absence of DIS3. Therefore, we show that DIS3 ribonuclease plays a critical role in germ cell meiosis during spermatogenesis in mice.

2021-07-16 | GSE180197 | GEO

RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-12-18 | GSE228454 | GEO