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Sediment metagenome

ABSTRACT: Lab-enriched sediment microbial nitrate-reducing benzene-degrading community from hydrocarbon-contaminated retail site in Toronto, Canada

PROVIDER: PRJNA951427 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
		Other
	SRR24043417_1.fastq.gz	Fastqsanger.gz
	SRR24043417_2.fastq.gz	Fastqsanger.gz
	SRR24043419_subreads.fastq.gz	Fastqsanger.gz

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Project description:Members of the bacterial phylum Spirochaetes are primarily studied for their commensal and pathogenic roles in animal hosts. However, Spirochaetes are also frequently detected in anoxic hydrocarbon-contaminated environments but their ecological role in such ecosystems has so far remained unclear. Here we provide a functional trait to these frequently detected organisms with an example of a sulfate-reducing, naphthalene-degrading enrichment culture consisting of a sulfate-reducing deltaproteobacterium Desulfobacterium naphthalenivorans and a novel spirochete Rectinema cohabitans. Using a combination of genomic, proteomic, and physiological studies we show that R. cohabitans grows by fermentation of organic compounds derived from biomass from dead cells (necromass). It recycles the derived electrons in the form of H2 to the sulfate-reducing D. naphthalenivorans, thereby supporting naphthalene degradation and forming a simple microbial loop. We provide metagenomic evidence that equivalent associations between Spirochaetes and hydrocarbon-degrading microorganisms are of general importance in hydrocarbon- and organohalide-contaminated ecosystems. We propose that environmental Spirochaetes form a critical component of a microbial loop central to nutrient cycling in subsurface environments. This emphasizes the importance of necromass and H2-cycling in highly toxic contaminated subsurface habitats such as hydrocarbon-polluted aquifers.

2018-05-17 | PXD005624 | Pride

Metatranscriptomics analysis of a benzene-degrading culture

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Project description:Effects of Chelators on Iron-reducing Benzene-Degrading Cultures

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Project description:Benzene-degrading bioreactor microbial communities from Toronto, Ontario, Canada, that are methanogenic - MIRA/Phrap_Assembly metagenome

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citrate vs nitrate Reducing cultures

Project description:The effect of nitrate reduction (anaerobic cultivation in the presence of heme, vitamin K2 and nitrate) was compared with anaerobic cultivation supplemented with citrate (Lactobacillus plantarum). The medium was chemically defined medium with mannitol as main carbon source Two-condition experiment, nitrate vs citrate reducing cells. Biological replicates: 4 nitrate reducing cultures, 4 citrate reducing cultures, independently grown and harvested. Two slides were used, each slide contained 8 Arrays. Citrate reducing cultures are called reactor 1-4, Nitrate reducing cultures are called reactor A-D

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