Ontology highlight
ABSTRACT:
PROVIDER: PRJNA951805 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24058106.fastq.gz | Fastqsanger.gz | |||
| SRR24058257.fastq.gz | Fastqsanger.gz | |||
| SRR24058557.fastq.gz | Fastqsanger.gz | |||
| SRR24056658.fastq.gz | Fastqsanger.gz | |||
| SRR24058377.fastq.gz | Fastqsanger.gz |
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Project description:Metronidazole (Mtz) is the frontline chemotherapeutic for a variety of anaerobic pathogens, including the gastroenterinal parasitic protist, Giardia duodenalis. However, treatment failure and clinical resistance is common and increasing. In Giardia, in vitro drug-resistant lines allow controlled experimental interrogation of resistance mechanisms in in vitro, isogenic cultures. These mechanisms centrally involve regulation of glycolytic and antioxidant oxidoreducatases, however this is inconsistent across isolate lines, likely incomplete, and further complicated by phenotypic plasticity in Giardia, which regains Mtz susceptibility after cessation of drug selection, or via passage through the life cycle. Global, quantitative approaches are required to reconstruct complex Mtz resistance phenotypes, resolve isolate variability, and explore unresolved hypotheses regarding post-translational modifications as the regulators of this phenotype. Our study addresses these gaps by comprehensively characterising proteomic changes in three seminal Mtz resistant lines compared to their isogenic, Mtz-susceptible, parental lines. Using quantitative proteomics, we have calculated differentially expressed proteins (DEPs) in MtzR lines, and probed via Western blot changes in protein acetylation, methylation, ubiquitination and phosphorylation post-translational modifications (PTMs). Finally, we have also performed the first controlled, longitudinal study
2018-02-21 | PXD007183 | Pride
Project description:Giardia duodenalis is a prevalent intestinal pathogens known to cause giardiasis, a condition characterized by diarrhea and frequently linked to malnutrition and growth impairments in children. The presence of Giardiavirus (GLV) in Giardia strains has been associated with heightened immune cytokine responses in the host compared to the GLV-free strains. However, the transmission mode and biological significance of GLV remain unclear. In this study, by using G. duodenalis DH (GLV-containing) and WBC6 (GLV-free) strains, we demonstrated that the DH strain produced extracellular vesicles (EVs), which originated from unique peripheral vesicle and bead-like structures in the ventrolateral flange. Nanoparticle tracking analysis revealed that GLV infected-G. duodenalis DH strain secreted fewer EVs than the GLV-free WBC6 strain. Biochemical and electron microscopy demonstrated that GLV virions can exploit the Giardia EVs pathway to facilitate their spread among parasites. Giardia uptake of GLV-containing EVs occured through clathrin-mediated endocytosis, leading to rapid infection of trophozoites by GLV. Furthermore, GLV infection enhanced messenger RNA translation efficiency, influencing protein abundance in Giardia trophozoites. The presence of GLV also upregulated the glycolytic pathway, with Giardia enolase closely associated with GLV replication. Importantly, Giardia infected with GLV alleviated the pathogenicity of parasite compared to the GLV-freeGiardia strain. These findings highlight the pivotal role of GLV in regulating Giardia biology, suggesting its potential for informing the development of novel intervention strategies against Giardia infections.
2024-12-31 | GSE269427 | GEO
Project description:Giardia duodenalis strain:WBC6 Raw sequence reads
| PRJNA1135215 | ENA
Project description:Giardia duodenalis strain:WB1B Raw sequence reads
| PRJNA298647 | ENA
Project description:Despite of Giardia duodenalis being one of the most commonly found intestinal pathogens in humans and animals, little is known of the host-parasite interactions in natural hosts. Therefore, the objective of this study was to investigate the intestinal response in calves following a G. duodenalis infection, using a bovine high-density oligo microarray to analyze global gene expression in the small intestine. The resulting microarray data suggested a decrease in inflammation, immune response and immune cell migration in infected animals, which was examined in more detail by quantitative real-time PCR on a panel of cytokines combined with histological analyses. The cytokine transcription levels showed a trend of down regulated expression in infected animals compared to the negative controls, best seen in jejunum for IL-6 and IL-8 and statistically significant for IL-17, IL-13 and IFN-?. No increased immune cell recruitment could be seen after infection, as well as no intestinal pathologies, such as villus shortening or increased levels of apoptosis. Key regulators in this intestinal response seem to be the nuclear peroxisome proliferator-activated receptors alpha (PPARA) and gamma (PPARG), for which an up-regulated expression was seen on microarray and qRT-PCR data. The activation of PPARs can exert an anti-inflammatory effect with inhibition of pro-inflammatory cytokines and a decrease in cell recruitment. . How the PPARs are activated during a Giardia infection still needs to be further elucidated. Eight male Holstein calves aged two to four weeks old were used for the trial. Prior to arrival, all animals were screened for the presence of Giardia cysts in their faecal samples. After confirming their negative status for all these pathogens, four of the animals were randomly chosen and placed in a G. duodenalis contaminated environment, whereas the four remaining animals were kept as negative controls in separate G. duodenalis-free stables. All calves in the study received the same commercial milk replacer. After three weeks, the presence or absence of a G. duodenalis infection was confirmed by IFA on faecal samples after which the animals were euthanized. Changes in gene expression profiles induced by Giardia duodenalis infection were compared using a high-density 60mer bovine oligo microarray.
2012-04-07 | E-GEOD-35920 | biostudies-arrayexpress