Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24116774_1.fastq.gz | Fastqsanger.gz | |||
| SRR24116774_2.fastq.gz | Fastqsanger.gz |
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Project description:we compared the skin transcriptomes of the black- and white-coated region from the Boer and Macheng Black crossbred goat with black head and white body using the Illumina RNA-Seq method. Six cDNA libraries derived from skin samples of the white coat region (n = 3) and black coat region (n = 3) were constructed from three full-sib goats. On average, we obtained approximately 76.5 and 73.5 million reads for each skin sample of black coat and white coat, respectively, of which 75.39% and 76.05% reads were covered in the genome database. Our study provides insight into the transcriptional regulation of two distinct coat color that might serve as a key resource for understanding coat color pigmentation of goat.
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Project description:The present study, for the first time, compared the transcriptomes of ovaries from the prolific Jintang black goat and the non-prolific Tibetan goat during follicular phase using the Illumina RNA-Seq method. The study provides insight into the transcriptional regulation in the ovaries of two distinct breeds of goats that might serve as a key resource for understanding goat fecundity.
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Project description:Laiwu black goat kid liver mRNA expression profile were sequenced with novaSeq 6000. The liver tissues were procured at 5-time points from the Laiwu black goats of 1 day, 2 weeks, 4 weeks, 8 weeks, and 12 weeks of age, respectively with 5 goats per time point, for a total of 25 goats.
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Project description:The growth and mature of Sertoli cells (SCs) are important part of testicular development and miRNAs are considered as novel regulatory molecules involved in it. Here we systematically screened and analyzed the key miRNAs participating in testicular development of black goat, as well as identifying underlying mechanism of candidate mRNAs contributes to spermatogenesis. The miRNA transcriptional profiles of testicular tissues at 3 days old (D3), 30 days old (D30), 90 days old (D90) and 180 days old (D180) were constructed and comparative analyzed. Results showed that the most differentially expressed (DE) miRNAs were found between the testicular tissues of D30 and D90 black goats. Moreover, the predicted target genes of DE miRNAs are mainly enriched in biological processes including cell proliferation, cell composition and enriched in signaling pathways including p53, FoxO and PI3K-Akt. The top one DE miRNA (miR-34c-5p), which significantly highly expressed in the testis of D90 when compared to that of D30, was mainly located within SCs and targeted to IGF1R. By performing miR-34c-5p overexpression and RNA interference, we found that the function of miR-34c-5p in SCs was inhibited cell proliferation, increased apoptosis rate, and significantly reduced the expression of growth factors (CXCL12, BMP4, GDNF and IGF1). In summary, our study suggests that miR-34c-5p can serve as a potential target for regulation of testicular development and a reference marker of sex maturation applicated in black goat genetic breeding.
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Project description:Establishment and maintenance of spermatogenesis need a complex process and vast regulatory network. There is growing evidence reveals that long noncoding RNAs (lncRNA) plays important role in regulating testicular development and spermatogenesis in a stage-specific way. We here report the identification of lncRNA LOC108635509 as key lncRNA regulator in black goat spermatogenesis. In the current study, we screened the transcriptomes (lncRNA and mRNA) of testicular from Guangxi black goats before puberty (3 days old, D3; 30 days old, D30), puberty (90 days old, D90) and postpuberty (180 days old, D180), and found there were 1211, 12180, 834 differential lncRNAs and 1196, 8838,269 differential mRNAs at the ages of D30 vs D3, D90 vs D30, and D180 vs D90. The expression pattern of differentially expressed (DE) lncRNAs indicated that D90 was a key node of lncRNAs participated in the regulation of testicular development and spermatogenesis in black goat. The of GO and KEGG analyses identified that DE lncRNAs and their target genes regulated spermatogenesis through MAPK, Ras, and PI3K-Akt signal pathways. Using cis- and trans-acting, 39 DE lncRNA-targeted genes were found to be enriched for male reproduction. Of these, LOC108635509, which specific expressed in testis and upregulated the expression levels at D90, was found participated in the regulation of testicular development through promoting the proliferation of SCs. Overall, this study provides new insight into the regulatory mechanisms that support spermatogenesis and testicular development in black goats.
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Project description:A black goat metatranscriptomics (PRJCA018033)
| PRJDB36183 | ENA