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Dual genetic lineage tracing reveals functional neuromesodermal progenitors

ABSTRACT: Dual genetic lineage tracing reveals functional neuromesodermal progenitors

PROVIDER: PRJNA952913 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24092257_1.fastq.gz	Fastqsanger.gz
	SRR24092257_2.fastq.gz	Fastqsanger.gz
	SRR24092258_1.fastq.gz	Fastqsanger.gz
	SRR24092258_2.fastq.gz	Fastqsanger.gz
	SRR24092259_1.fastq.gz	Fastqsanger.gz

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Project description:Background: Phenotypic plasticity of smooth muscle cells (SMCs) and endothelial cells (ECs) contributes to atherosclerotic plaque composition and stability, yet how shifts in one population influence the contribution and function of the other under conditions of vascular stress, such as irradiation, is poorly understood. A major limitation has been the inability to simultaneously fate map both cell types within the same lesion, with most studies mapping one lineage while inferring the other using unreliable dynamically changing marker genes, risking false-positive and false negative assignment. Methods: We generated dual lineage-tracing Apoe-deficient mice, enabling simultaneous fate mapping of SMCs and ECs. This model was used to extend prior findings from single lineage tracing models demonstrating irradiation-induced loss of SMC lesion investment and expansion of EC-derived cells. Dual lineage-tracing mice were subjected to irradiation followed by Western diet feeding to induce atherosclerosis. Lineage tracing, immunostaining and scRNA-seq analysis were used to define coordinated SMC and EC responses and identify changes relevant to plaque instability. Results: Dual lineage tracing simultaneously labeled SMC- and EC-derived cells in healthy and atherosclerotic vessels. Irradiation induced divergent responses: SMC-derived cells failed to invest in lesions and upregulated stress-activated inflammatory genes, whereas EC-derived cells expanded and upregulated SMC-associated genes. However, EC-derived cells within lesions failed to induce extracellular matrix genes, and lesions from irradiated mice exhibited reduced collagen content and fewer ACTA2+ cells within the fibrous cap, consistent with reduced plaque stability. Conclusions: Dual lineage-tracing of SMCs and ECs demonstrated that irradiation-induced loss of lesional SMC and expansion of EC-derived ACTA2+ cells are not artifacts of false lineage assignment. By resolving SMC and EC fate within the same lesion, we identify irradiation-induced cell dynamics including inflammatory reprogramming of SMCs, EC phenotypic modulation, impaired extracellular matrix organization, and reduced ACTA2⁺ fibrous cap cellularity that may contribute to radiotherapy-associated increased atherosclerotic cardiovascular disease risk. Key Words: Atherosclerosis; smooth muscle cells; endothelial cells; lineage tracing; radiation; extracellular matrix; cellular plasticity

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Dual genetic lineage tracing reveals functional neuromesodermal progenitors

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