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Porcine circovirus modulates swine influenza virus replication in pig trachea epithelial cells and porcine alveolar macrophages

ABSTRACT: Porcine circovirus modulates swine influenza virus replication in pig trachea epithelial cells and porcine alveolar macrophages

PROVIDER: PRJNA953411 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24107200.fastq.gz	Fastqsanger.gz
	SRR24107201.fastq.gz	Fastqsanger.gz
	SRR24107179.fastq.gz	Fastqsanger.gz
	SRR24107180.fastq.gz	Fastqsanger.gz
	SRR24107181.fastq.gz	Fastqsanger.gz

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Porcine circovirus modulates swine influenza virus replication in pig trachea epithelial cells and porcine alveolar macrophages [NPTr]

Project description:The pathogenesis of porcine circovirus type 2b (PCV2b) and swine influenza A virus (SwIV) during co-infection in swine respiratory cells is poorly understood. To elucidate the impact of PCV2b/SwIV co-infection, newborn porcine tracheal epithelial cells (NPTr) and immortalized porcine alveolar macrophages (iPAM 3D4/21) were co-infected with PCV2b and SwIV (H1N1 or H3N2 genotype). Viral replication, cell viability and cytokine mRNA expression were determined and compared between single-infected and co-infected cells. Finally, 3’mRNA sequencing was performed to identify the modulation of gene expression and cellular pathways in co-infected cells. It was found that PCV2b significantly decreased and improved SwIV replication, in co-infected NPTr and iPAM 3D4/21 cells respectively, compared to single infected cells. Interestingly, PCV2b/SwIV co-infection synergistically up-regulated IFN expression in NPTr cells whereas in iPAM 3D4/21 cells, PCV2b impaired the SwIV IFN induced response, both correlating with SwIV replication modulation. RNA-sequencing analyses revealed that the modulation of gene expression and enriched cellular pathways during PCV2b/SwIV H1N1 co-infection is regulated in a cell type-dependent-manner. This study revealed different outcomes of PCV2b/SwIV co-infection in porcine epithelial cells and macrophages and provides new insights on porcine viral co-infections pathogenesis.

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Porcine circovirus modulates swine influenza virus replication in pig trachea epithelial cells and porcine alveolar macrophages [iPAM]

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Porcine circovirus modulates swine influenza virus replication in pig trachea epithelial cells and porcine alveolar macrophages [iPAM]

Project description:Porcine circovirus modulates swine influenza virus replication in pig trachea epithelial cells and porcine alveolar macrophages [iPAM]

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Project description:Porcine circovirus modulates swine influenza virus replication in pig trachea epithelial cells and porcine alveolar macrophages [NPTr]

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Genome-wide transcriptional response of primary alveolar macrophages (PAMs) following infection with porcine circovirus type 2

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Genome-wide transcriptional response of primary alveolar macrophages (PAMs) following infection with porcine circovirus type 2

Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Porcine circovirus type 2 (PCV2) has been identified as the causal agent of postweaning multisystemic wasting syndrome, an economically important multifactorial disease of the swine industry worldwide. We used microarrays to study the transcriptome of PAMs infection with PCV2. PAMs were collected by bronchoalveolar lavage from health piglets (free of PCV2, PRRSV, PRV, CSFV, PPV), and PAMs were cultured for 48 hours and inoculated with 5 moi of PCV2.

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Comparison of the transcriptome response within the tracheobronchial lymph node following infection with Porcine Reproductive and Respiratory Syndrome Virus, Porcine Circovirus 2 or Swine Influenza A Virus.

Project description:The aim of this study was to acquire a better understanding of porcine reproductive and respiratory syndrome (PRRS) disease through a deeper knowledge of gene expression changes that occur in pulmonary lymph nodes by comparing PRRS virus (PRRSV), porcine circovirus type 2 (PCV-2), and swine influenza virus (IAV-S) infections. The PRRSV, IAV-S and PCV-2 viral infections followed a clinical course in these domestic pigs typical of experimental infection of young pigs with these viruses. PRRSV isolate SDSU-73 was pathogenic in this study inducing fever, anorexia, listlessness, and dyspnea.

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