Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

BMDMs were transfected with RFP or U1 and then infected with HSV-1 or not

ABSTRACT: BMDMs were transfected with RFP or U1 and then infected with HSV-1 or not

PROVIDER: PRJNA953941 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

BMDMs were transfected with RFP or U1 and then infected with HSV-1 or not

Project description:Test transcripts in mock and HSV-1 infected BMDMs which transfected with RFP or U1 snRNA

2025-09-09 | GSE229274 | GEO

The interacting proteins of U1 snRNA in the HSV-1-infected BMDMs

Project description:We used biotinylated RNA pull-down combined with mass spectrometry to screen the interacting proteins of U1 snRNA in the HSV-1-infected BMDMs.

| PXD068204 |

Effect of U1 snRNA knock-in on gene expression during HSV-1 infection

Project description:To investigate the effect of U1 snRNA knock-in on gene expression during HSV-1 infection, we obtained BMDMs from WT and U1 snRNA myeloid knock-in (U1mKI) mice, and infected them with HSV-1 (MOI, 1) for 4 hours. Subsequently, we performed gene expression profiling analysis using RNA-seq.

2025-09-09 | GSE269901 | GEO

Effect of in vitro-transcribed U1 snRNA on gene expression

Project description:To investigate the effect of in vitro-transcribed U1 snRNA on gene expression, we transfected A549 cells with in vitro-transcribed U1 snRNA or control RFP (200 ng/mL). We then performed gene expression profiling analysis using RNA-seq.

2025-09-09 | GSE270055 | GEO

Effect of depletion of Grina on gene expression during HSV-1 infection of BMDMs

Project description:To investigate the role of Grina in HSV-1-induced signaling pathways, we isolated total RNA from Grina-knockout (Grina-KO) BMDMs and their wild-type (WT) controls, with or without HSV-1 infection. Bone marrow-derived macrophages were generated from Grina-floxed (Grina-fl/fl) and Lyz2-Cre-mediated Grina-KO mice, and were mock-infected or infected with HSV-1 (MOI = 1) for 4 hours. Total RNA was extracted from four biological replicates per condition, representing four experimental groups: WT mock, WT HSV-1, KO mock, and KO HSV-1. This dataset enables comprehensive identification of Grina-dependent transcriptional responses during HSV-1 infection, providing insights into the crosstalk between Grina-mediated signaling and antiviral innate immunity.

2026-09-01 | GSE345297 | GEO

Sorted MDMs with RFP+GFP+ or RFP+GFP- Mtb

Project description:Human serum derived macrophages were infected with Mtb expressing a transcriptional reporter of viability and on day 5, the cells were sorted for RFP+GFP+ macrophages or RFP+GFP- macrophages.

2019-06-10 | GSE131220 | GEO

Sorted MDMs with RFP+GFP+ or RFP+GFP- Mtb

Project description:Sorted MDMs with RFP+GFP+ or RFP+GFP- Mtb

| PRJNA542934 | ENA

Exogenous expression analysis of U1 snRNA mutation

Project description:To reveal the effect of U1 snRNA mutation, we performed exogenous expression analysis of U1 snRNA mutation. The pLKO.1-puro U6 sgRNA BfuAI stuffer lentiviral vector was modified by removing the internal U6 promoter. It was replaced by the U1 locus, including 393 bases of internal native U1 promoter, the U1 sequence, and 39 bases of 3’-flanking region. The r.3A>G mutation was introduced by site-directed mutagenesis. HEK-293T cells were transfected using Lipofectamine Plus with either pLKO.1-U1wt or pLKO.1-U1r.3a>g in duplicate. Messenger RNA library construction was performed based on oligo dT-based mRNA isolation using NEBNext® Poly(A) mRNA Magnetic Isolation Module. RNA Sequence was performed on NextSeq 550 using 100-bp paired-end mode.

2019-10-09 | GSE128005 | GEO

Effect of U1 snRNA knock-in on gene expression during HSV-1 infection

Project description:Effect of U1 snRNA knock-in on gene expression during HSV-1 infection

| PRJNA1124088 | ENA

ribo-seq in control and U1 AMO treated HeLa cells

Project description:Control and U1 AMO were transfected into Hela cells, Ribosome-associated RNAs were purified and sequenced in Novaseq platform.To compare the translation efficiency, mRNA-seq were performed in parallel.

2023-02-27 | GSE193200 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data