Project description:We used biotinylated RNA pull-down combined with mass spectrometry to screen the interacting proteins of U1 snRNA in the HSV-1-infected BMDMs.
Project description:To investigate the effect of U1 snRNA knock-in on gene expression during HSV-1 infection, we obtained BMDMs from WT and U1 snRNA myeloid knock-in (U1mKI) mice, and infected them with HSV-1 (MOI, 1) for 4 hours. Subsequently, we performed gene expression profiling analysis using RNA-seq.
Project description:To investigate the effect of in vitro-transcribed U1 snRNA on gene expression, we transfected A549 cells with in vitro-transcribed U1 snRNA or control RFP (200 ng/mL). We then performed gene expression profiling analysis using RNA-seq.
Project description:Human serum derived macrophages were infected with Mtb expressing a transcriptional reporter of viability and on day 5, the cells were sorted for RFP+GFP+ macrophages or RFP+GFP- macrophages.
Project description:To reveal the effect of U1 snRNA mutation, we performed exogenous expression analysis of U1 snRNA mutation. The pLKO.1-puro U6 sgRNA BfuAI stuffer lentiviral vector was modified by removing the internal U6 promoter. It was replaced by the U1 locus, including 393 bases of internal native U1 promoter, the U1 sequence, and 39 bases of 3’-flanking region. The r.3A>G mutation was introduced by site-directed mutagenesis. HEK-293T cells were transfected using Lipofectamine Plus with either pLKO.1-U1wt or pLKO.1-U1r.3a>g in duplicate. Messenger RNA library construction was performed based on oligo dT-based mRNA isolation using NEBNext® Poly(A) mRNA Magnetic Isolation Module. RNA Sequence was performed on NextSeq 550 using 100-bp paired-end mode.
Project description:Control and U1 AMO were transfected into Hela cells, Ribosome-associated RNAs were purified and sequenced in Novaseq platform.To compare the translation efficiency, mRNA-seq were performed in parallel.
Project description:We found that the germline transcription factor double homeobox 4 (DUX4) is upregulated upon infection with wild-type herpes simplex virus-1 (HSV-1). The goal of this experiment was to compare the cellular transcriptome of HEK293T cells that were infected with HSV-1 (KOS strain), or transfected with a plasmid encoding human DUX4.