Theileria annulata
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR32482046_1.fastq.gz | Fastqsanger.gz | |||
| SRR32482046_2.fastq.gz | Fastqsanger.gz | |||
| SRR32482047_1.fastq.gz | Fastqsanger.gz | |||
| SRR32482047_2.fastq.gz | Fastqsanger.gz |
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Project description:This intracellular parasite causes East Coast Fever in cattle
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Project description:This intracellular parasite causes East Coast Fever in cattle
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Project description:The apicomplexan parasite Theileria parva (Tp) causes East Coast Fever (ECF), an often fatal tick-borne disease especially for Bos taurus cattle breeds that causes substantial economic losses for cattle farmers in sub-Saharan Africa. Replacement of the currently employed live vaccine with a more standardised, stable, affordable, and cross-protective subunit vaccine has not yet been successful and requires the identification of additional suitable parasite antigens. Carbohydrate-based molecules play an important role in the survival and establishment of pathogens including protozoan parasites and can represent strong virulence factors. The current knowledge of the glycobiology of Tp, however, is very limited which led us to investigate the glycosylation potential of Tp in this study. Screening of isolated Tp Muguga (TpM) schizonts with C-type lectin (-like) receptors identified recognition of the parasites by ruminant but not murine Macrophage C-type lectin (MCL). This suggested the presence of carbohydrate-associated schizont surface molecules. Specific binding of isolated schizonts by WGA but not ConA further suggested the presence of terminal GlcNAc residues on the surface of the parasites which is in alignment with the identification of a minimal N-glycosylation machinery in TpM schizonts. LC-MS/MS analyses of either HILIC-HPLC or WGA enriched peptides from parasite protein fractions identified eight predicted N-glycopeptides and two predicted O-glycopeptides. While putative parasite O-glycans were assigned with high confidence, assignments of putative N-glycans to parasite proteins were less confident. Confirmation of both the assignment and the composition of predicted parasite glycans requires further analyses.
2026-06-29 | PXD070746 | Pride
Project description:Theileria orientalis is a tick-borne apicomplexan protozoan parasite of cattle.
| PRJNA49583 | ENA
Project description:Tropical theileriosis in a cattle disease of global economic importance, caused by the tick-borne protozoan parasite Theileria annulata. Conventional control strategies are failing to contain the disease and an attractive alternative is the use of pre-existing genetic resistance or tolerance. However, tropical theileriosis tolerant cattle are less productive than some susceptible breeds. To combine resistance and production traits requires an understanding of the mechanisms involved in resistance. Therefore, we have compared the response of monocytes derived from tolerant (Sahiwals, Bos indicus) and susceptible (Holstein-Friesians, B. taurus) cattle to in vitro infection with T. annulata. Over 150 genes exhibited breed-specific differential expression during the course of infection and nearly one third were differentially expressed in resting cells, implying that there are inherent differences between monocytes from the breeds. Fifty sequences currently only match ESTs or are unique to the library used to generate the microarray. The differential expression of a selection of genes was validated by quantitative RT-PCR, e.g. CD9, prion protein and signal-regulatory protein alpha. A large proportion of the differentially expressed genes encode proteins expressed on the plasma membrane or in the extracellular space and cell adhesion was one of the major Gene Ontology biological processes identified. We therefore hypothesise that the breed-specific tolerance of Sahiwal cattle compared to Holstein-Friesians is due to the interaction of infected cells with other immune cells, which influences the immune response generated against T. annulata infection. The BoMP microarray is available from the ARK-Genomics facility (www.ark-genomics.org).
2007-04-26 | E-MAXD-25 | biostudies-arrayexpress
Project description:Extracellular vesicles (EVs) are key mediators of intercellular communication, and often play critical roles in host-parasite interactions by facilitating parasite’s physiology and pathogenesis. Theileria annulata, an apicomplexan parasite, induces profound changes in host cells, leading to uncontrolled proliferation, apoptosis resistance, and increased invasiveness. In this study, we performed the comprehensive proteomic and small RNA analysis of EVs isolated from a T. annulata Kashi isolate-infected bovine lymphocyte cell line (TaXJS), B cell line (TaBC), dendritic cell line (TaDC), and from the sera of cattle before and after infection. Our label-free LC-MS/MS proteomics identified 2580 proteins, while small RNA sequencing revealed 6635 miRNAs associated with parasite development, host invasion, and immune evasion. Functional enrichment analyses recognized vesicular components involved in key pathways of the parasite-host such as ECM-receptor interaction, oxidative phosphorylation, and proton transport. These findings highlight the potential of Theileria-derived EVs in modulating host responses and their potential as therapeutic and vaccine targets.
2025-10-21 | GSE279977 | GEO
Project description:Extracellular vesicles (EVs) are key mediators of intercellular communication, and often play critical roles in host-parasite interactions by facilitating parasite’s physiology and pathogenesis. Theileria annulata, an apicomplexan parasite, induces profound changes in host cells, leading to uncontrolled proliferation, apoptosis resistance, and increased invasiveness. In this study, we performed the comprehensive proteomic and small RNA analysis of EVs isolated from a T. annulata Kashi isolate-infected bovine lymphocyte cell line (TaXJS), B cell line (TaBC), dendritic cell line (TaDC), and from the sera of cattle before and after infection. Our label-free LC-MS/MS proteomics identified 2580 proteins, while small RNA sequencing revealed 6635 miRNAs associated with parasite development, host invasion, and immune evasion. Functional enrichment analyses recognized vesicular components involved in key pathways of the parasite-host such as ECM-receptor interaction, oxidative phosphorylation, and proton transport. These findings highlight the potential of Theileria-derived EVs in modulating host responses and their potential as therapeutic and vaccine targets.
2025-09-29 | PXD056906 | Pride
Project description:Intracellular pathogens develop elaborate mechanisms to survive within the hostile environments of host cells. Theileria parasites infect bovine leukocytes and cause devastating diseases in cattle in developing countries. Theileria spp. have evolved sophisticated strategies to hijack host leukocytes, inducing proliferative and invasive phenotypes characteristic of cell transformation. Intracellular Theileria parasites secrete proteins into the host cell and recruit host proteins to induce oncogenic signaling for parasite survival. It is unknown how Theileria parasites evade host cell defense mechanisms, such as autophagy, to survive within host cells. Here, we show that Theileria annulata parasites sequester the host eIF5A protein to their surface to escape elimination by autophagic processes. We identified a small-molecule compound that reduces parasite load by inducing autophagic flux in host leukocytes, thereby uncoupling Theileria parasite survival from host cell survival. We took a chemical genetics approach to show that this compound induced host autophagy mechanisms and the formation of autophagic structures via AMPK activation and the release of the host protein eIF5A which is sequestered at the parasite surface. The sequestration of host eIF5A to the parasite surface offers a strategy to escape elimination by autophagic mechanisms. These results show how intracellular pathogens can avoid host defense mechanisms and identify a new anti-Theileria drug that induces autophagy to target parasite removal.
2023-12-19 | GSE250088 | GEO