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Genetic and Clinical Determinants of Telomere Length

ABSTRACT: Genetic and Clinical Determinants of Telomere Length

PROVIDER: PRJNA956671 | ENA |

REPOSITORIES: ENA

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Genetic and Clinical Determinants of Telomere Length

Project description:Genetic and Clinical Determinants of Telomere Length

| PRJNA956672 | ENA

Telomere variant sequences encode a genetic blueprint for allele-specific telomere length (dataset 2)

Project description:The ends of human chromosomes are capped by specialized nucleoprotein structures known as telomeres, which are essential for genome stability. Recent advances in long-read sequencing have enabled allele-specific telomere length measurements at nucleotide resolution, uncovering extreme heterogeneity in telomere length between alleles that is determined at birth and gradually shortens with age. The shortening over time makes steady-state telomere length in human somatic cells a promising biomarker for age-associated diseases. However, the mechanisms underlying allele-specific telomere maintenance and its stability remain poorly understood. Here, we developed a high-resolution workflow combining PacBio and Nanopore long-read sequencing platforms to map allele-specific telomere length in human blood samples as well as cultured cell lines. By tracing allele-specific telomeric sequence in family members across multiple generations, we show that the allele-specific telomeric variant sequences (TVSs) are heritable (with mean similarity score > 0.95) and underlie the extreme heterogeneity of telomere length between alleles. Continuous cell proliferation likely drives the slow but stochastic evolution of allele-specific TVSs distribution, resulting in asymmetry in telomere inheritance from father and mother (p-value = 2.354e-7). Targeted deletion of allele-specific TVSs using CRISPR-Cas9 resets telomere length, further confirming their causal role in the control of allele-specific telomere maintenance (p-value < 2e-6). These results indicated that TVSs encode a novel class of heritable genetic elements underlying the allele-specific telomere length.

2026-07-10 | GSE328480 | GEO

Telomere variant sequences encode a heritable genetic blueprint for allele-specific telomere length (dataset 1)

Project description:The ends of human chromosomes are capped by specialized nucleoprotein structures known as telomeres, which are essential for genome stability. Recent advances in long-read sequencing have enabled allele-specific telomere length measurements at nucleotide resolution, uncovering extreme heterogeneity in telomere length between alleles that is determined at birth and gradually shortens with age. The shortening over time makes steady-state telomere length in human somatic cells a promising biomarker for age-associated diseases such as clonal hematopoiesis. However, the mechanisms underlying allele-specific telomere maintenance and its stability remain poorly understood. Here, we developed a high-resolution workflow combining PacBio and Nanopore long-read sequencing platforms to map allele-specific telomere length in human blood samples as well as telomerase-positive cancer cell lines. By tracing allele-specific telomeric sequence in family members across multiple generations, we show that telomeric variant sequences (TVSs) interspersed throughout the canonical repeat region are heritable (with mean similarity score > 0.95), allele-specific, and account for the extreme heterogeneity of telomere length between alleles. Targeted deletion of allele-specific TVSs using CRISPR-Cas9 resets telomere length, further confirming their causal role in the control of allele-specific telomere maintenance (p-value < 3e-29). Continuous cell proliferation likely drives the slow but stochastic evolution of allele-specific TVSs distribution, resulting in asymmetry in telomere inheritance from father and mother (p-value < 0.045). These results indicated that TVSs encode a novel class of heritable genetic elements underlying the allele-specific telomere length.

2026-07-10 | GSE311938 | GEO

Telomere variant sequences encode a genetic blueprint for allele-specific telomere length (dataset 2)

Project description:Telomere variant sequences encode a genetic blueprint for allele-specific telomere length (dataset 2)

| PRJNA1455523 | ENA

Telomere length effect on genes near the telomere in myoblasts

Project description:We examined differential expression of genes within 10MBs of telomeres in myoblasts with long or short telomeres We offer telomere looping with telomere length as a partial mechanistic explanation for the changes gene expression that is observed. Compare expression of genes within 10MB of the telomere in normal myoblasts with long (15 kb) and short (6 kb) telomeres.

2014-07-17 | E-GEOD-48973 | biostudies-arrayexpress

Telomere variant sequences encode a heritable genetic blueprint for allele-specific telomere length (dataset 1)

Project description:Telomere variant sequences encode a heritable genetic blueprint for allele-specific telomere length (dataset 1)

| PRJNA1371950 | ENA

Telomere length effect on genes near the telomere in myoblasts

2014-07-17 | GSE48973 | GEO

Subtelomeric DNA methylation controls telomere length

Project description:DNAm control of telomere length

| PRJEB110807 | ENA

Genetic and clinical determinants of telomere length.

Project description: Not available

| S-EPMC10199259 | biostudies-literature

Telomere heterogeneity linked to metabolism and pluripotency state revealed by simultaneous measurement of telomere length and RNA-seq in the same human ES cell

Project description:Telomere length heterogeneity in various cell types including stem cells and cancer cells has been recognized. Cell heterogeneity also is found in pluripotent stem cells such as embryonic stem cells (ESCs). The implication and mechanisms underlying the heterogeneity remain to be defined. We have optimized a robust method that can simultaneously measure telomere length coupled with RNA-sequencing analysis (scT&R-seq) in the same human ES cell. Using this method, we show that telomere length varies with pluripotency state. Long telomere hESCs highly express TERF1/TRF1 as well as ZFP42/REX1, PRDM14 and NANOG for naïve pluripotency, in contrast to short telomere hESCs. hESCs express high telomerase activity as expected, and ubiquitously express NOP10 and DKC1, stabilizing components of telomerase complexes, regardless of telomere lengths. Moreover, new candidate genes such as MELK, MSH6 and UBQLN1 cluster with long telomeres and pluripotency network. Notably, short telomere hESCs exhibit higher oxidative phosphorylation primed for lineage differentiation, whereas long telomere hESCs show elevated glycolysis, another key feature for naïve pluripotency. Our data further suggest that telomere length is implicated in metabolism activity and pluripotency state of hESCs. Single cell analysis of telomere and RNA-sequencing can be exploited to further understand the molecular mechanisms of telomere heterogeneity.

2017-11-06 | GSE98644 | GEO

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