Project description:Accurate differentiation between closely related Haemophilus species is essential for clinical diagnosis. This study presents a high-resolution proteotyping workflow integrating whole-cell MALDI-TOF MS, nanoLC-MS/MS, and comparative genomics to distinguish H. influenzae and H. aegyptius. We identified 31 robust protein biomarkers validated by LC-MS/MS peptide mapping and comparative genomics, providing enhanced diagnostic resolution for closely related pathogens.
Project description:Annotation of small RNAs from 11 Drosophila species for the purpose of non-coding RNA annotation and comparative genomics assessment.