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BRAFV600E-induced transcriptional changes in cord blood-derived hematopoietic stem and progenitor cells

ABSTRACT: BRAFV600E-induced transcriptional changes in cord blood-derived hematopoietic stem and progenitor cells

PROVIDER: PRJNA957261 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24211946.fastq.gz	Fastqsanger.gz
	SRR24211947.fastq.gz	Fastqsanger.gz
	SRR24211948.fastq.gz	Fastqsanger.gz
	SRR24211949.fastq.gz	Fastqsanger.gz
	SRR24211950.fastq.gz	Fastqsanger.gz

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BRAFV600E-induced transcriptional changes in cord blood-derived hematopoietic stem and progenitor cells

Project description:BRAFV600E is a recurrent mutation in the histiocytoic disorders Langerhans cell histiocytosis (LCH) and Erdheim Chester Disease (ECD). The mutation can be detected in committed monocytes/macrophages, dendritic cells, and hematopoietic stem and progenitor cells (HSPCs). Although the cell of origin that gives rise to these disorders is currently unknown, it was recently suggested that LCH and ECD arise from BRAFV600E-mutant HSPCs. To understand the underlying molecular mechanisms in HSPCs driving this phenotype, we introduced BRAFV600E into healthy human HSPCs using CRISPR/Cas9.

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