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ABSTRACT: Kluyveromyces marxianus strain sequencing

PROVIDER: PRJNA957743 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR24224991_subreads.fastq.gz	Fastqsanger.gz
	SRR24224992_subreads.fastq.gz	Fastqsanger.gz

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Project description:Kluyveromyces marxianus is a non-conventional yeast with outstanding physiological characteristics and a high potential for lignocellulosic ethanol production. However, achieving high ethanol produc-tivities implies overcoming several biotechnological challenges as the cellular inhibition caused by the inhibitors present in the medium. In this work, the adaptation of K. marxianus SLP1 to increase the tolerance to a mix of inhibitory compounds was carried out using the adaptive laboratory evolution (ALE) strategy. As a result of the ALE adaptation process, an improved K. marxianus strain (P8) was obtained after 8 serial passes. The fermentative and physiological parameters evidenced a better response of the P8 strain against the synergistic effect of multiple inhibitors. The P8 strain reduced the lag phase from 12 to 4 h, increasing 40% the biomass and improving 16-fold the volumetric eth-anol productivity. To test the transcriptional dynamics for the adaptation process, we performed a differential gene expression analysis in control conditions; the results showed that the basal gene expression in P8 changes, suggesting the biological capability of K. marxianus to activate the ad-aptative prediction mechanism. This study demonstrates the rapid adaptability of K. marxianus SLP1 to stressful environments, making this yeast a promising candidate to produce lignocellulosic ethanol.

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