Ontology highlight
ABSTRACT:
PROVIDER: PRJNA957971 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24261000_1.fastq.gz | Fastqsanger.gz | |||
| SRR24261000_2.fastq.gz | Fastqsanger.gz | |||
| SRR24261001_1.fastq.gz | Fastqsanger.gz | |||
| SRR24261001_2.fastq.gz | Fastqsanger.gz | |||
| SRR24261002_1.fastq.gz | Fastqsanger.gz |
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Project description:<p>Background The gut microbiota plays a significant role in modulating the growth and function of host muscle, and microbiota transplantation experiments provide compelling evidence of its capacity to improve muscle quality. Feeding faba beans improves the muscle quality of Yellow River carp. However, the changes in gut microbiota, along with the specific microorganisms, metabolic pathways, and regulatory mechanisms linked to the enhancement of muscle quality following faba bean consumption remain to be elucidated.</p><p>Results After a 6-week feeding trial with faba beans, growth performance decreased, but muscle texture improved (P < 0.05). Gut microbiota structure also changed, with increased relative abundances of Aeromonas, ZOR0006, Cetobacterium, and Atopobium. Following 8 weeks of whole-intestinal microbiota transplantation (WIMT) from faba bean-fed donors to basal diet-fed recipients of Yellow River carp, growth performance remained unchanged (P > 0.05), while muscle texture improved (P < 0.05). This improvement was mainly due to increased small-diameter muscle fibers, higher collagen levels, and reduced muscle fat content (P < 0.05), which partially replicated the muscle texture of donor fish. Moreover, WIMT promotes intestinal structure and barrier integrity, with significant changes in gut microbiota structure and metabolic profile. WIMT improved the muscle quality of Yellow River carp by regulating mitochondrial autophagy and adipocytokine signaling pathways through the gut-muscle axis. Cetobacterium somerae (C. somerae) and its metabolites, such as acetic acid, played a crucial role in this process. Further feeding experiments demonstrated that C. somerae and acetic acid reduced the crude fat content of muscle while increasing the crude protein and collagen (P < 0.05). C. somerae also mitigated muscle protein degradation under inflammatory and enhanced collagen (P < 0.05), thereby improving muscle texture.</p><p>Conclusion This study establishes that gut microbiota enhance muscle quality in Yellow River carp through WIMT, identifies C. somerae and its metabolite acetic acid as key contributors. The findings provide novel evidence for fish gut-muscle axis research and offer new scientific basis for improving Yellow River carp muscle quality.</p>
2025-10-13 | MTBLS13128 | MetaboLights
Project description:n order to investigate the molecular mechanisms underlying locomotion differences between two common carp (Cyprinus carpio) strains cultured in a rice-fish symbiosis system, we conducted comparative proteomic analyses of muscle tissues collected from indolent Congjiang (CJ) and active FFRC No. 2 (FR) strains cultured under the identical paddy field conditions.
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Project description:Intestinal flora of Crucian carp sequencing
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Project description:<p>The large yellow croaker (LYC, <em>Larimichthys crocea</em>) is highly regarded for its delicious taste and unique flavor. The gut microbiota has the ability to affect the host muscle performance and elasticity by regulating nutrient metabolism. However, the mechanism by which the intestinal microbiota regulates the muscle elasticity of LYC remains poorly understood. In this study, the intestinal contents of high muscle elasticity males (IEHM), females (IEHF), and low muscle elasticity males (IELM) and females (IELF) were collected and subjected to metagenomic and metabolomic analyses. Metagenomic sequencing results showed that the intestinal flora structures of LYCs with different muscle elasticities were significantly different. The abundance of Streptophyta in the IELM ( 24.63%) and IELF (29.68%) groups was significantly higher than that in the IEHM and IEHF groups. The abundance of Vibrio scophthalmi (66.66%) in the IEHF group was the highest. Based on metabolomic analysis by liquid chromatograph-mass spectrometry, 107 differentially abundant metabolites were identified between the IEHM and IELM groups, and 100 differentially abundant metabolites were identified between the IEHF and IELF groups. Based on these metabolites, a large number of enriched metabolic pathways related to muscle elasticity were identified. Significant differences in intestinal metabolism between groups with different muscle elasticities were identified. Moreover, the model of the relationship between the intestinal flora and metabolites was constructed, and the molecular mechanism of intestinal flora regulation of nutrient metabolism was further revealed. The results help to understand the molecular mechanism of different muscle elasticities of LYC and provide an important reference for the study of the mechanism of the effects of LYC intestinal symbiotic bacteria on muscle development, and the development and application of probiotics in LYC.</p>
2025-07-31 | MTBLS10457 | MetaboLights
Project description:This study is conducted to test the hypothesis that patients with lung cancer undergoing treatment with immunotherapy, chemotherapy and tyrosine Kinase Inhibitors receiving a high protein diet and supplemented with fish oil will improve muscle mass, therapeutic response and modulate the intestinal microbiota within a 4 month period.
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Project description:To explore the regulatory mechanism of intestinal flora in Citrobacter rodentium -induced intestinal infection by transcriptome analysis at miRNA molecular level.
2024-03-04 | GSE260446 | GEO
Project description:<p> Introduction: Muscle growth and development in Liaoning cashmere goats (a dual-purpose breed for cashmere and meat) and its regulation by intestinal flora are key to improving meat production, yet relevant molecular mechanisms remain unclear.</p><p> Objectives: To reveal the molecular mechanism of muscle growth and development in Liaoning cashmere goats and the regulatory role of intestinal flora, thereby improving their meat production performance.</p><p> Methods: Muscle tissues (intercostal, hindlimb biceps femoris, longissimus dorsi) and digestive contents (rumen, small intestine, cecum, feces) from goats at 6, 12, 18 months were collected. Transcriptomics, proteomics, metabolomics, 16S rRNA sequencing, and multi-omics integration analysis were used.</p><p> Results: HBFM exhibited optimal development at 12 months of age, which was influenced by g_Lachnospiraceae_NK3A20_group in the small intestine. The HOXC10 gene drove myocyte proliferation, while the MYL3 gene and its encoded protein synergistically participated in muscle physiological changes, involving the cAMP signaling pathway. LDM development was more active at 12 months, regulated by g_Christensenellaceae_R-7_group in the rumen and cecum, leading to significant expression of the MEF2A gene and MYH6 protein. Additionally, the COL1A1/COL1A2 genes participated in intramuscular fat deposition through purine metabolism. ICM showed vigorous early proliferation at 6 months, regulated by g_Aeriscardovia in the small intestine, which promoted high expression of Actin. L-malic acid and L-serine affected muscle growth and water-holding capacity in this region. At 18 months, the GDI protein influenced fat deposition in ICM through the cofactor biosynthesis pathway. Multi-omics integration analysis revealed that microorganisms such as g_norank_f_UCG-011 were significantly associated with numerous metabolites, and genes like FKBP5 synergistically regulated muscle development with proteins such as Actin.</p><p><em>Conclusion:</em> The development of different muscle parts in Liaoning cashmere goats is coordinately regulated by specific genes, proteins, metabolites, and intestinal flora, providing key targets for targeted breeding to improve meat production performance.</p>
2025-10-30 | MTBLS12972 | MetaboLights