Candida albicans
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA96131 | ENA |
REPOSITORIES: ENA
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Project description:Candida albicans is a common resident of humans that colonizes multiple body sites in most healthy individuals (e.g., the gut), but can rapidly proliferate and initiate specific microbial programs to cause infections in immunocompromised hosts. However, the molecular mechanisms underlying its commensal-pathogenic switch have not been fully elucidated. Here, we demonstrate that the mitochondrial protein Mcu1, associated with TCA cycle enzymes, plays a crucial role in sensing N-acetylglucosamine (GlcNAc), a key host intestinal signal, and regulates phenotypic switching in C. albicans. Disruption of Mcu1 or key TCA cycle enzymes impairs the ability of mutant strains to utilize GlcNAc as a carbon source, blocks white-opaque switching in vitro, and reduces intestinal colonization in vivo. We further demonstrated that Mcu1 modulates the mitochondrial redox state, which thereby influences respiratory metabolism, including TCA cycle. Furthermore, Mcu1 regulates white-GUT switching by regulating the expression of Wor1, the master transcriptional regulator of white-opaque switching. Since GUT cells represent a colonization-specific morphology that supports commensalism, our findings indicate that Mcu1 and TCA cycle enzymes cooperatively regulate the commensal-pathogenic switch in C. albicans.
2026-01-20 | PXD071309 | Pride
Project description:pH regulates white-opaque switching and sexual mating in Candida albicans
| PRJNA278957 | ENA
Project description:As a successful commensal and pathogen of humans, Candida albicans encounters a wide range of environmental changes. Among them, ambient pH is an important factor, which changes frequently and affects many biological processes in this species. The ability to adapt to pH changes is tightly linked with pathogenesis and morphogenesis. In this study, we report that pH has a profound effect on white-opaque switching and sexual mating in C. albicans. Acidic pHs promote white-to-opaque switching but repress sexual mating of opaque cells. The cAMP signaling and Rim101-mediated pH sensing pathways are involved in the regulation of pH-regulated white-opaque switching. Interestingly, white and opaque cells of the cyr1/cyr1 mutant, which is defective in producing cAMP, show distinct growth defects under acidic and alkaline conditions. Phr2 could play a major role in acidic pHs-induced opaque cell formation. We further discover that acidic pH conditions repress sexual mating due to the failure of activation of the Ste2-mediated a-pheromone response pathway. The effects of pH changes on phenotypic switching and sexual mating could be a balance behavior between host adaptation and sexual reproduction.
2015-12-30 | GSE67101 | GEO
Project description:ofr1 regulates white-to-opaque switching and mating of Candida albicans (MTLa/α)
| PRJNA305349 | ENA
Project description:The discovery of white-opaque switching in natural MTLa/alpha isolates of Candida albicans sheds new light on the evolution of phenotypic plasticity and host adaptation.
2013-04-01 | GSE43938 | GEO
Project description:The human pathogen Candida albicans can assume either of two distinct cell types, designated ‘‘white’’ and ‘‘opaque.’’ Each cell type is maintained for many generations; switching between them is rare and stochastic, and occurs without any known changes in the nucleotide sequence of the genome. The two cell types differ dramatically in cell shape, colony appearance, mating competence, and virulence properties. In this work, we investigate the transcriptional circuitry that specifies the two cell types and controls the switching between them. First, we identify two new transcriptional regulators of white-opaque switching, Czf1 and white-opaque regulator 2 (Wor2). Analysis of a large set of double mutants and ectopic expression strains revealed genetic relationships between CZF1, WOR2, and two previously identified regulators of white-opaque switching, WOR1 and EFG1. Using chromatin immunoprecipitation, we show that Wor1 binds the intergenic regions upstream of the genes encoding three additional transcriptional regulators of white-opaque switching (CZF1, EFG1, and WOR2), and also occupies the promoters of numerous white- and opaque-enriched genes. Based on these interactions, we have placed these four genes in a circuit controlling white-opaque switching whose topology is a network of positive feedback loops, with the master regulator gene WOR1 occupying a central position. Our observations indicate that a key role of the interlocking feedback loop network is to stably maintain each epigenetic state through many cell divisions. Keywords: ChIP-chip
2007-10-01 | GSE8709 | GEO
Project description:Interlocking Transcriptional Feedback Loops Control White-Opaque Switching in Candida albicans
| PRJNA101945 | ENA
Project description:Ssn6, a new regulator of white-opaque switching in Candida albicans
| PRJNA248763 | ENA
Project description:In Candida albicans, the a1-alpha2 complex represses white-opaque switching as well as mating. A ChIP-chip strategy was, therefore, used to screen for genes with a1-alpha2 binding sites and expression patterns consistent with a master switch gene (MSG). Of 51 genes identified with an a1-alpha2 binding site, one gene, TOS9, also referred to as EAP2, exhibited an expression pattern consistent with a MSG. TOS9 is expressed in opaque, not white a/a and alpha/alpha cells and Tos9p localizes to the opaque cell nucleus. Deletion of TOS9 blocks cells in the white phase, and misexpression in the white phase of the parent tos9+/tos9+ strain results in mass conversion to opaque. Expression of TOS9 under control of a MET promoter rescues the TOS9 null mutant tos9-/tos9- phenotype. Temperature-induced mass conversion of opaque to white in the parent strain results in the immediate cessation of TOS9 transcription, and loss of Tos9p prior to the point of commitment to white (the switch event), which occurs at the time of the second cell doubling. Misexpression of TOS9, as well as inhibition of the second round of DNA replication inhibits the temperature-induced switch from opaque to white. Based on these observations, a model is developed for the regulation and role of TOS9 in switching. Keywords: ChIP chip
2006-09-19 | GSE5493 | GEO
Project description:The discovery of white-opaque switching in natural MTLa/alpha isolates of Candida albicans sheds new light on the evolution of phenotypic plasticity and host adaptation. Comparing gene expression of white and opaque cells of a MTL a/alpha strain
2013-04-01 | E-GEOD-43938 | biostudies-arrayexpress