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Comparative RNA-seq analysis of Arabidopsis thaliana wild-type vs. autophagy atg5 mutant under water deprivation.

ABSTRACT: Comparative RNA-seq analysis of Arabidopsis thaliana wild-type vs. autophagy atg5 mutant under water deprivation.

PROVIDER: PRJNA962896 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24339364_1.fastq.gz	Fastqsanger.gz
	SRR24339364_2.fastq.gz	Fastqsanger.gz
	SRR24339365_1.fastq.gz	Fastqsanger.gz
	SRR24339365_2.fastq.gz	Fastqsanger.gz
	SRR24339366_1.fastq.gz	Fastqsanger.gz

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Comparative RNA-seq analysis of Arabidopsis thaliana wild-type vs. autophagy atg5 mutant under water deprivation.

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Project description:Purpose: Global survey of the transcriptome in autophagy-deficient strains of D. discoideum Results: Our RNAseq analysis of ATG5‾, ATG5‾/12‾ and ATG5‾/12‾/16‾ cells revealed major transcriptional changes in a large number of genes in comparison to AX2 Conclusions: Our study presents a first detailed analysis of the transcriptome in autophagy-deficient strains of D. discoideum. The data were generated by RNA-seq technology. The optimised data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA content within a cell. We conclude that RNA-seq based transcriptome characterisation would expedite genetic network analyses and permit the dissection of complex biologic functions.

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| MSV000090348 | MassIVE

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