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TET2-mediated mRNA in leukemia stemness maintanence and homing [RNA-Seq]

ABSTRACT: TET2-mediated mRNA in leukemia stemness maintanence and homing [RNA-Seq]

PROVIDER: PRJNA963840 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24358319_1.fastq.gz	Fastqsanger.gz
	SRR24358319_2.fastq.gz	Fastqsanger.gz
	SRR24358320_1.fastq.gz	Fastqsanger.gz
	SRR24358320_2.fastq.gz	Fastqsanger.gz
	SRR24358321_1.fastq.gz	Fastqsanger.gz

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TET2-mediated mRNA in leukemia stemness maintanence and homing

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TET2-mediated mRNA in leukemia stemness maintanence and homing [RNA-Seq]

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TET2-mediated mRNA in leukemia stemness maintanence and homing

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TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewal.

Project description:TET2 is recurrently mutated in acute myeloid leukemia (AML) and its deficiency promotes leukemogenesis (driven by aggressive oncogenic mutations) and enhances leukemia stem cell (LSC) self-renewal. However, the underlying cellular/molecular mechanisms have yet to be fully understood. Here, we show that Tet2 deficiency significantly facilitates leukemogenesis in various AML models (mediated by aggressive or less aggressive mutations) through promoting homing of LSCs into bone marrow (BM) niche to increase their self-renewal/proliferation. TET2 deficiency in AML blast cells increases expression of Tetraspanin 13 (TSPAN13) and thereby activates the CXCR4/CXCL12 signaling, leading to increased homing/migration of LSCs into BM niche. Mechanistically, TET2 deficiency results in the accumulation of methyl-5-cytosine (m5C) modification in TSPAN13 mRNA; YBX1 specifically recognizes the m5C modification and increases the stability and expression of TSPAN13 transcripts. Collectively, our studies reveal the functional importance of TET2 in leukemogenesis, leukemic blast cell migration/homing, and LSC self-renewal as an mRNA m5C demethylase.

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Project description:To investigate the function of TET2 as an mRNA m5C demethylase in the development of acute myeloid leukemia (AML), we conducted RNA bisulfite sequencing of mRNA samples enriched from AML cells carrying Tet2+/+ versus Tet2-/- with actinomycin D treatment at different time point.

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Context Dependent Effects of Ascorbic Acid Treatment in TET2 Mutant Myeloid Neoplasia

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