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N6-methyladenosine modification is not a general trait of viral RNA genomes [CHIKV]

ABSTRACT: N6-methyladenosine modification is not a general trait of viral RNA genomes [CHIKV]

PROVIDER: PRJNA967595 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24443287_1.fastq.gz	Fastqsanger.gz
	SRR24443287_2.fastq.gz	Fastqsanger.gz
	SRR24443288_1.fastq.gz	Fastqsanger.gz
	SRR24443288_2.fastq.gz	Fastqsanger.gz
	SRR24443289_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

N6-methyladenosine modification is not a general trait of viral RNA genomes [Dengue]

Project description:N6-methyladenosine (m6A), the most common internal RNA modification in eukaryotic mRNAs, is described to be abundantly present in the genomes of cytoplasmic-replicating RNA viruses. Yet, how the host nuclear m6A writer has access to the viral RNAs in the cytoplasm and what are the associated biological consequences remain unknown. Here, we comprehensively addressed these questions by combining antibody-dependent (m6A-seq) and antibody-independent (SELECT and nanopore direct RNA sequencing) methods on the cytoplasmic-replicating Chikungunya virus (CHIKV) RNA, and found no evidence of m6A modifications. Moreover, depletion of m6A modification machinery components did not affect CHIKV infection, and CHIKV infection did not alter their cellular localization. Consistent with these observations, no m6A modifications were found in the RNA genome of the dengue virus (DENV), another cytoplasmic-replicating virus. Our results challenge the idea that m6A modification is a general trait of cytoplasmic-replicating RNA viruses and stress the need of confirming antibody-dependent detection of m6A modifications with orthogonal antibody-independent methods.

2024-01-23 | GSE231738 | GEO

N6-methyladenosine modification is not a general trait of viral RNA genomes [CHIKV]

2024-01-23 | GSE231737 | GEO

N6-methyladenosine modification is not a general trait of viral RNA genomes.

Project description:N6-methyladenosine modification is not a general trait of viral RNA genomes.

| PRJNA967585 | ENA

N6-methyladenosine modification is not a general trait of viral RNA genomes

Project description:N6-methyladenosine modification is not a general trait of viral RNA genomes (MinION runs)

| PRJEB61652 | ENA

N6-methyladenosine modification is not a general trait of viral RNA genomes [Dengue]

Project description:N6-methyladenosine modification is not a general trait of viral RNA genomes [Dengue]

| PRJNA967597 | ENA

N6-methyladenosine modification is not a general trait of viral RNA genomes (Plasmid Illumina runs)

Project description:N6-methyladenosine modification is not a general trait of viral RNA genomes (Plasmid Illumina runs)

| PRJEB66350 | ENA

N6-methyladenosine modification is not a general trait of positive-sense RNA viral genomes

Project description:<p>Despite the nuclear localization of the m6A machinery, the genomes of multiple exclusively-cytoplasmic RNA viruses, such as chikungunya (CHIKV) and dengue (DENV), are reported to be extensively m6A-modified. However, these findings are mostly based on m6A-seq, an antibody-dependent technique with a high rate of false positives. Here, we addressed the presence of m6A in CHIKV and DENV RNAs. For this, we combined m6A-seq and the antibody-independent SELECT and nanopore direct RNA sequencing techniques with functional, molecular, and mutagenesis studies. Following this comprehensive analysis, we found no evidence of m6A modification in CHIKV or DENV transcripts. Furthermore, depletion of key components of the host m6A machinery did not affect CHIKV or DENV infection. Moreover, CHIKV or DENV infection had no effect on the m6A machinery’s localization. Our results challenge the prevailing notion that m6A modification is a general feature of cytoplasmic RNA viruses and underscore the importance of validating RNA modifications with orthogonal approaches.</p>

2024-01-25 | MTBLS6978 | MetaboLights

N6-methyladenosine modification is not a general trait of viral RNA genomes.

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-01-23 | GSE231739 | GEO

N6-methyladenosine (m6A) profiling in 60 YRI LCLs

Project description:N6-methyladenosine (m6A), the most abundant messenger RNA (mRNA) modification, has emerged as a critical post-transcriptional mechanism that regulates various aspect of mRNA metabolism including pre-mRNA processing, mRNA export, mRNA stability and translation efficiency. However the genetics basis of m6A is largely unexplored. In this work, we mapped m6A-QTLs in 60 LCLs and used the resulting tens of thousands of QTLs to provide insights to m6A biology and its contribution to human complex trait.

2020-05-04 | GSE125377 | GEO

N6-Methyladenosine methylation profile in rat H9C2 cardiomyocytes under the hypoxic stress

Project description:Hypoxia as a crucial pathogenesis factor usually results in huge harmful effects on cardiac injury and dysfunction. In our previous study (PMID: 33294289), We observe a series of differential expressed genes between transcription and translation, which may be attributed to the hypoxia-specific binding affinity of Nuclear cap-binding subunit 3 (NCBP3) at 5’ un-translation region of target genes. But the underlying molecular mechanism of NCBP3 for gene translation modulation remains unclear. Here, we conducted RIP-seq of N6-Methyladenosine methylation in H9C2 cells with the conditions of normoxic, hypoxic and with additional NCBP3 knockdown.

2021-06-30 | E-MTAB-10682 | biostudies-arrayexpress

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