Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Central amygdala cell types and their transcriptional regulation by fasting

ABSTRACT: Central amygdala cell types and their transcriptional regulation by fasting

PROVIDER: PRJNA967687 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24446010_1.fastq.gz	Fastqsanger.gz
	SRR24446010_2.fastq.gz	Fastqsanger.gz
	SRR24446011_1.fastq.gz	Fastqsanger.gz
	SRR24446011_2.fastq.gz	Fastqsanger.gz
	SRR24446012_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 44

Similar Datasets

Central amygdala cell types and their transcriptional regulation by fasting

Project description:A complete cell type census based on their full molecular profiles at subclass level and their subnuclei distribution in the central amygdala (CeA), linked with their behavior relevance, is still lacking. Here, we performed single nuclei RNA sequencing in adult mice and compared the results with known genetic and functionally defined populations. We identified 9 transcriptionally and spatially discrete cell types (3 in the lateral division CeL; 5 in the medial division CeM; and 1 in the capsular division CeC). In the CeL, we found one PKCδ expressing cluster (CeL PKCδ ) and two clusters marked by Sst: the CeL Sst cluster which contains the largest and most highly expressing population of Sst-positive neurons, and the CeL NTS/Tac2 cluster which contains low expressing Sst-positive cells. This two Sst population are also Htr2a positive. The CeC contains one cluster marked by the expression of Calcrl and partially expressed PKCδ. In the CeM, the largest cluster was marked by Il1Rapl2, which contains the CeM subsets of Pnoc and Htr2a, and is likely appetitive. Another Sst population, the CeM Tac1/Sst, remains largely unexplored. Moreover, we discovered three previously uncharacterized CeM clusters: CeM Vdr, CeM Drd2/Rai14, and CeM Dlk1. To begin investigating whether these cell types show individual responses in appetitive behavior, we compared their transcriptomes in food-deprived and satiated mice. We found a cell type specific transcriptional response across neuronal populations, particularly in one of the appetitive clusters in the CeL.

2023-05-06 | GSE231790 | GEO

Gene expression of central amygdala of mice

Project description:Purpose: The goals of this study are to screen out the changes of gene expression profile in response to high fat diet, an mRNA-seq was performed in central amygdala Methods: 6-week-old male wild-type mice were fed with high fat diet (HFD, containing 45% fat) or normal chow diet (ND) for 5 months. And then their central amygdala tissues were removed and total RNA was extracted to perform RNA sequencing. Results:The KEGG pathway analysis showed that genes related to neuroactive ligand-receptor interaction and calcium signaling pathway were dramatically upregulated after HFD Conclusions: Our study represents the first detailed analysis of central amygdalar transcriptomes of mice subjected to high fat diet, with biologic replicates, generated by RNA-seq technology. Our results show genes related to neuroactive ligand-receptor interaction and calcium signaling pathway were dramatically upregulated after high fat diet feeding,which hint at the importance of ion channel in central amygdala after high fat feeding.

2022-02-17 | GSE196719 | GEO

Gene expression of central amygdala of mice

Project description:Gene expression of central amygdala of mice

| PRJNA806964 | ENA

Central Amygdala TRAP-Seq of Drd2 neurons

Project description:Central Amygdala TRAP-Seq of Drd2 neurons

| PRJNA472614 | ENA

Single cell RNA-sequencing in the mouse central amygdala (CEA)

Project description:The central amygdala (CEA) is a brain region that consists of primarily GABAergic neurons. It has been widely investigated for its role in many innate and adaptive behaviors, such as appetitive and defensive behaviors. Despite the complex functions of CEA, the molecular diversity of CEA neurons has not been systemically examined. Here, we performed single-cell RNA-sequencing (scRNA-Seq) in the CEA to classify the molecularly defined neuron types in this region.

2023-02-03 | GSE213828 | GEO

Transcriptomic landscape of the central amygdala of the mouse

Project description:Inhibitory neurons are the predominant type of neuron in the mouse central amygdala. Here, we examined diversity in this cell type using single-cell RNA-seq.

2022-10-19 | GSE184666 | GEO

Transcriptional Idenetity of Itch-activated Central Amygdala Neurons

Project description:Transcriptional Idenetity of Itch-activated Central Amygdala Neurons

| PRJNA534481 | ENA

A central extended amygdala circuit that modulates anxiety

Project description:A central extended amygdala circuit that modulates anxiety

| PRJNA454720 | ENA

Molecular profiling of insular cortex neurons projecting to the central amygdala

Project description:We used the retro-TRAP protocol (Ekstrand et al 2014) to profile insular cortex neurons that project to the central amygdala

2020-12-24 | GSE163743 | GEO

RNA-Seq of post mortem human central Amygdala samples in patients affected by opioid use disorder (OUD) vs. unaffected control samples

Project description:Differential expression analysis of human central Amygdala samples with a story of opioid use disorder (OUD) vs. unaffected control central Amygdala samples

2022-01-28 | GSE194368 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data