Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differences in gene expression of liver cancer cells after co-culture with activated CD8+ T cells in vitro

ABSTRACT: Differences in gene expression of liver cancer cells after co-culture with activated CD8+ T cells in vitro

PROVIDER: PRJNA970660 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24476314_1.fastq.gz	Fastqsanger.gz
	SRR24476314_2.fastq.gz	Fastqsanger.gz
	SRR24476315_1.fastq.gz	Fastqsanger.gz
	SRR24476315_2.fastq.gz	Fastqsanger.gz
	SRR24476316_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Differences in gene expression of liver cancer cells after co-culture with activated CD8+ T cells in vitro

Project description:To investigate the difference in gene expression of liver cancer cells after co-culture with activated CD8+T cells , we established a tumor cells/activated CD8+T cells co-culture system in which murine liver cancer cell line hepa1-6 were co-cultured and attacked directly by murine in vitro activated spleen naive CD8+T cells (effector: target=5:1) for 48 hours. We then performed gene expression profiling analysis using data obtained from RNA-seq of two different groups (immune attacked or control) with 5 repetitions per group.

2024-07-25 | GSE232024 | GEO

Co-Culture of CD8+ T cells and Breast Cancer Cells

Project description:Targeting of tumor immune escape mechanisms holds enormous therapeutic potential. Still, most patients progress under immune checkpoint blockade and some even become hyper-progressors. To investigate how cancer cells respond to activated but ineffective T cells, we challenged peptide-loaded MCF-7 breast cancer cells with antigen-specific CD8+ T cells in which Perforin had been destroyed by pre-treatment with Concanamycin A.

2019-11-25 | GSE108236 | GEO

Gene expression of resting memory, resting naive and in vitro activated memory CD8+CD127+ T cells from spleen and bone marrow (BM)

Project description:To understand differences between resting and activated memory CD8+ T cells, we compared the global gene expression of ex vivo isolated naive and spleen and BM memory cells to in vitro activated spleen and BM memory cells.

2015-02-05 | GSE62691 | GEO

Transcriptional response of HIV-infected CD4 memory T cells to co-culture with TCR activated CD8 T cells.

Project description:Transcriptional response of HIV-infected CD4 memory T cells to co-culture with TCR activated CD8 T cells.

| PRJNA815755 | ENA

Effect of STAT3-/+ secretome in CD8+ T cells in vitro culture

Project description:To investigate the effect of the addition of STAT3-/+ astrocyte derived-conditioned medium in CD8+ T cells previously activated in vitro We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 CD8+ T cells cultures of STAT3- conditon and 3 CD8+ T cells cultures of STAT3+ conditon

2024-08-30 | GSE228364 | GEO

Transcriptomic differences between deeply quiescent and activated adult NSCs

Project description:Transcriptomic differences between deeply quiescent and activated adult NSCs

| PRJNA999746 | ENA

Retinal Pigment Epithelial Cells Upregulate Expression of Complement Factors after Co-culture with Activated T Cells

Project description:In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration.

2011-02-02 | GSE17938 | GEO

H3K79me2 naïve and in vitro activated B cells

Project description:H3K79me2 naïve and in vitro activated B cells

| PRJNA577712 | ENA

Chemokine expression in retinal pigment epithelial cells in response to co-culture with activated T Cells

Project description:Purpose: To investigate the effects of T cell-derived cytokines on gene and protein expression of chemokines in a human RPE cell line (ARPE-19). Methods: We used an in vitro co-culture system in which the RPE and CD3/28-activated T cells were separated by a membrane. Differential gene expression in the RPE cells of chemokine genes was quantified using three different microarrays. Protein expression was determined by single- and multiplex ELISA and immunoblotting. Results: Co-culture with activated T cells increased RPE mRNA and protein expression of chemokines CCL2 (MCP-1), CCL5 (RANTES), CCL7 (MCP-3), CCL8 (MCP-2), CXCL1 (GRO-α), IL8 (CXCL8), CXCL9 (MIG), CXCL10 (IP10), CXCL11 (ITAC), and CX3CL1 (fractalkine). The secretion of CCL7 and CXCL9, 10, and 11 was polarized in the apical direction. Using recombinant human cytokines and neutralizing antibodies we identified IFNgamma and TNFalpha as the two major T cell-derived cytokines responsible for the RPE response. For CCL5, CXCL9, 10, 11, 16, and CX3CL1 we observed a synergistic effect of IFNgamma and TNFalpha in combination. CCL20, CXCL1 and 6, and IL8 were negatively regulated by IFNgamma. Conclusions: RPE cells responded to exposure to T cell-derived cytokines by upregulating expression of several chemokines related to microglial, T cell, and monocyte chemotaxis and activation. This inflammatory stress response may have implications for immune homeostasis in the retina, and for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration. Differentiated ARPE19 grown on inserts in serum-free media was basolaterally stimulated for 48h with activated T cells or recombinant cytokines (IFNg and/or TNFa) or neutralizing antibodies (aIFNg and/or aTNFa)

2012-11-19 | E-GEOD-36331 | biostudies-arrayexpress

Gene expression differences between activated B cells expressing GL7 and not in Peyer´s patches

Project description:Gene expression differences between activated B cells expressing GL7 and not in Peyer´s patches

| PRJNA530140 | ENA