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RNA-sequencing reveals differentially expressed rice genes functionally associated with defense against brown planthopper in NILs pyramiding BPH14 and BPH15

ABSTRACT: RNA-sequencing reveals differentially expressed rice genes functionally associated with defense against brown planthopper in NILs pyramiding BPH14 and BPH15

PROVIDER: PRJNA971999 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24529400_1.fastq.gz	Fastqsanger.gz
	SRR24529400_2.fastq.gz	Fastqsanger.gz
	SRR24529401_1.fastq.gz	Fastqsanger.gz
	SRR24529401_2.fastq.gz	Fastqsanger.gz
	SRR24529402_1.fastq.gz	Fastqsanger.gz

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RNA-sequencing reveals differentially expressed rice genes functionally associated with defense against brown planthopper in NILs pyramiding BPH14 and BPH15

Project description:Brown planthopper (BPH) is one of the most destructive pests in rice production. The pyramiding application of BPH-resistance genes BPH14 and BPH15 can effectively improve rice resistance to BPH, however, the molecular mechanisms underlying BPH14/BPH15 pyramiding lines are poorly understood. Here, a mRNA expression profiling analysis was performed on the near isogenic lines (NILs) containing the BPH14, BPH15 or BPH14/BPH15 and their recurrent parent (RP) Wushansimiao. A total of 14492 differentially expressed mRNAs (DEGs) were identified from 12 mRNA profiles of resistant NILs and RP at different feeding stages. In the transcriptome analysis, 531 DEGs appeared to be common among the resistant NILs compared to RP before and after BPH feeding, which were enriched in defense response, phosphorylation and salt stress response. In addition, 258 DEGs shared only in resistant NILs were obtained among the different feeding stages, which were enriched in oxidative stress response, karrikin response and chloroplast organization. 21 DEGs were further selected as candidates for BPH resistance. OsPOX8.1, a potential candidate DEG related to BPH resistance, increased reactive oxygen species levels in rice protoplast. Our results provide valuable information to further explore the defense mechanism of insect-resistant gene pyramiding lines and develop robust strategies for insect control.

2023-07-03 | GSE232449 | GEO

Identification and analysis of microRNAs responsible for brown planthopper resistance in BPH14/BPH15 pyramiding rice

Project description:Background Rice farming faces a serious challenge from the brown planthopper (BPH), with the pyramiding of BPH14 and BPH15 genes delivering effective protection in elite rice strains. However, the molecular basis behind this resistance is still unclear. Results The study investigated miRNA levels in BPH14/BPH15 pyramiding line (B1415) and their recurrent parent (RP) under BPH infestation employing high-throughput sequencing and revealed 136 differentially expressed miRNAs (DEMs) among 550 known miRNAs. An integrated analysis highlighted that 587 miRNA-mRNA pairs linking 95 DEMs to 537 targeted genes were enriched in phenylpropanoid and lignin metabolism, circadian rhythms, and amino acid metabolism. The candidate DEMs, miR172d-3p, and miR396 family members were identified as negative regulators to decrease their target genes Os06g0708700 (encoding a nodulin-like protein) and Os11g0129700 (encoding an AP2 domain transcription factor), suggesting their key roles in rice against BPH. Conclusions Our investigation provides the first insights into miRNA-mediated defense mechanisms in the B1415. Identifying miRNAs and their target mRNAs in BPH resistance opens a new avenue for rice breeding programs, offering potential targets for improving pest resistance. Understanding these molecular interactions paves the way for developing more resistant rice cultivars, thereby contributing to sustainable rice production and food security.

2024-10-02 | GSE278202 | GEO

Brown planthopper

Project description:Brown planthopper Raw sequence reads

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Expression data from rice after brown planthopper attack

Project description:The aim of this study was to analyze potential brown planthopper (BPH) resistant genes in Rathu Heenati (RHT) by Affymetrix whole rice genome array,BPH susceptible and resistant rice varieties of TN1（Taichung Native 1）as control. All the resistant related genes derived from RHT will be analyzed according to the SSR markers interval flanked on the chromosome 3, 4, 6 and 10. It will be benefit to the gene clone and marker assistant breeding for Bph3 gene in the near future. We used microarrays to detail the global differential gene expression before and after brown planthopper attack in two different varieties, and identified distinct classes of high enriched genes induced by BPH or constituent in RHT

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Project description:Laodelphax striatellus breed:planthopper | cultivar:small brown planthopper Transcriptome or Gene expression

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Project description:Laodelphax striatellus breed:planthopper | cultivar:small brown planthopper Transcriptome or Gene expression

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