Project description:The study aimed the effects of Sasa quelpaertensis leaf hot water extract (SQH) on anti-fatigue function and the global gene expression pattern in muscle tissue. When rat skeletal muscle cells were cultured in hypoxic condition, the levels of lactate and lactate dehydrogenase (LDH) was increased approximately more than 2-fold, but SQH decreased effectively these levels. Thus, we assessed the exercise and fatigue recovery function using several physical biomarkers such as glycogen, lactate and so on in high intensity exercise mice. The mice were divided into three groups; Normal Control (NC), Exercise Control (EC), and Exercise SQH (ES) by oral administered at 50mg/kg/day for 7 days. After excessive swimming,the ES group showed increases serum glucose and decrease serum lactate than EC group. Lactate content in muscle was lower ES group than EC group. Glycogen contents in muscle and liver were higher ES group than EC group. And, SQH administration increased the expression of phospho-Acetyl-CoA (p-ACC), and reduced the expression of phospho-AMP-activated protein kinase (p-AMPK) and GLUT4 in in-vivo model. Also, genome wide expression profiles of tissue were determined by RNA sequencing. SQH up-regulates and down-regulates several genes associated with fatigue. In conclusion, this study suggested that SQH are effective for anti-fatigue agent.
Project description:The coordination of chloroplast and nuclear genome status are critical for plant cell function, but the mechanism remain largely unclear. In this study, we report that Arabidopsis thaliana CHLOROPLAST AND NUCLEUS DUAL-LOCALIZED PROTEIN 1 (CND1) maintains genome stability in both the chloroplast and the nucleus.
Project description:In the unicellular cyanobacterium, Synechococcus elongatus PCC 7942, most genes show rhythmic expression controlled by the Kai-based clock under continuous light conditions (LL). We found that rpoD6-null mutants impaired expression of clock-controlled genes peaking at hours 8-10 in LL, while sasA-null or rpaA-null mutants each arrested the expression profiles at subjective dawn. Time-course data (0-24 h) of wild type (WT), rpoD6-null, sasA-null and rpaA-null S. elongatus PCC 7942 strains analyzed under continuous light (LL) conditions after two 12h:12h light:dark (LD) cycles using Affymetrix high-density oligonucleotide microarrays (GeneChip CustomExpress Arrays) representing 2,515 predicted protein-coding genes on the genome of Synechococcus elongatus PCC 6301, which can be used also for the almost homologous strain, S. elongatus PCC 7942.
Project description:Complete chloroplast genome of the Korean endemic plant Semiaquilegia quelpaertensis (Isopyreae, Ranunculaceae) and phylogenetic analysis
Project description:The coordination of chloroplast and nuclear genome status are critical for plant cell function, but the mechanism remain largely unclear. In this study, we report that Arabidopsis thaliana CHLOROPLAST AND NUCLEUS DUAL-LOCALIZED PROTEIN 1 (CND1) maintains genome stability in both the chloroplast and the nucleus.
Project description:The regulator for chloroplast biogenesis (rcb) mutant was identified as a mutant defective in phytochrome-mediated chloroplast biogenesis. The rcb mutant has long hypocotyl and albino phenotypes. RCB initiates chloroplast biogenesis in the nucleus by promoting the degradation of the master repressors for chloroplast biogenesis, the PIFs (Phytochrome Interacting Factors). To understand how RCB regulates the expression of PIF-regulated genes, we performed genome-wide expression analysis of RCB-dependent genes using a rcb-10 null allele.