Project description:Baby leaves of lettuce (Lactuca sativa L.) are widely used to produce ready-to-eat salads all around the year. For this purpose, lettuce plants are cultivated under plastic tunnels and harvested at a very early growth stage, between 30-45 days after sowing depending on the season, cultivar and site of cultivation. Usually, the plastic film covering the cultivation tunnels transmits only a very small amount of the solar UVB radiation and partially attenuates UVA radiation. We used UV treatment post-harvest of fresh-cut lettuce baby leaves to compare the efficiency of two UV emission wavebands centred at 306 and 366 nm (achieved by employing narrow-band UV LEDs sources) to identify putative transcriptomic signatures. The analyses have been carried out supported by and in the frame of Project @CNR 2021, Title "EnhAncement of Natural anTIviral Compounds in ready- to-eat Vegetables", Acronym: ANTI-CoV to Dr. G. Agati. IDP-NAHEP, ICAR, Govt of India to A.R.B.
Project description:There are 18 ready-to-eat food samples (8 ready-to-eat meat, 7 ready-to-eat vegetables and 3 ready-to-eat fruit) and 6 human faecal samples. The samples were sequenced on the Illumina, NovaSeq PE150.
Project description:Listeria monocytogenes is an important food-borne pathogen which is frequently linked to ready-to-eat vegetables. In this work it was investigated by RNA-seq how the colonization of corn salad leaves and the commonly used packaging material polystyrene impacts on the transcriptome of L. monocytogenes NCTC 10887 at 4 °C and 22 °C. The strongest transcriptional differences occurred on corn salad at 4 °C versus polystyrene at 4 °C, yielding 2,629 differentially expressed genes (91.6% of protein-coding genes), indicating a habitat driven shift under cold-chain conditions. Corn salad promoted regulation of virulence related genes. The prfA gene was upregulated on corn salad compared to polystyrene at both temperatures (log2FC 2.43 at 22 °C and 4.16 at 4 °C), accompanied by increased hly (2.70), inlA (4.46), and inlB (2.10) expression at 4 °C. Stress/proteostasis gene expression was elevated on corn salad, including higher transcripts of clpP (3.34–3.51), clpE (5.58–6.89), and clpB (2.08–3.74), consistent with a stressed yet persistence-prone state on leaf surfaces. This indicates that L. monocytogenes persisting on leafy greens can remain transcriptionally primed for host interaction at 4 °C, implying that refrigeration alone may not mitigate risk on ready-to-eat produce, underlining the importance of pre-harvest hygiene.
2026-03-16 | GSE324553 | GEO
Project description:Occurrence & Characterization of Staphylococus aureus from Ready-to-eat Food in Singapore: A Retrospective Analysis
Project description:Global gene expression analysis of Staphylococcus aureus following Para-Tertiary Amyl Phenol (PTAP) treatment using Affymetrix GeneChip arrays. Results from this study provide insight into the molecular mechanisms underlying the cellular response of Staphylococcus aureus to PTAP.