Project description:Human lung cancer cell line A549 and H322m were subjected to cyclic strain (the frequency set in 15%, 0.5HZ) on 0h, 4h and 24h. We compared invasion assay, migration assay, colony formation and microarray assay in stretching and non-stretching groups.
Project description:RNA-seq analysis was performed in a TAL1-FKBP12 Jurkat cell line to analyze gene expression changes after dTAG-13 treatmentat at various time points (1h, 2h, 4h, 6h, 8h, 16h, 24h, 48h and 72h).
Project description:This study provides the dectin-1 and NFAT responsive genes for 2h and 4h of curdlan treatment. Affymetrix Mouse Gene 1.0ST Arrays were used to profile gene expression in curdlan-stimulated D1 cells (2h or 4h exposure) that had been treated or not with FK506 for the duration of culture.
Project description:This study provides the dectin-1 and NFAT responsive genes for 2h and 4h of curdlan treatment. Affymetrix Mouse Gene 1.0ST Arrays were used to profile gene expression in curdlan-stimulated D1 cells (2h or 4h exposure) that had been treated or not with FK506 for the duration of culture. Dendritic cell line D1 was treated with curdlan for 2h and 4h, and curdlan + Fk506 for 2h and 4h in three replicate.
Project description:The purpose of this work was to identify the potential signatures of cobalt exposure using a toxicogenomic approach. A time course transcriptome analysis was performed on human type II epithelial cell line A549. Cells were exposed to cobalt at midlog phase ; a medium without FCS containing cobalt (CoCl2, Sigma) or not was added for 30mn, 2h, 4h or 24h. Total RNA was isolated using the Quiagen RNeasy miniprep kit, and then labelled using the FairPlay Microarray Labelling Kit (Stratagene). Amino reactive Cy3- and Cy5- dyes (CyTMDye Post-Labelling Reactive Dye Pack, Amersham) were further chemically coupled to test and control cDNA respectively. After hybridization, microarrays were scanned with GenePix 4000B (Axon Instrument Inc., Forster City, CA). Cy3 and Cy5 fluorescence intensities for the spots were quantified after local subtraction of background noise using Genepix Pro 4.0 software (Axon Instrument Inc.) 30 mn : exposure 1 (2 microarrays) - exposure 2 (4 microarrays) 2h : exposure 1 (2 microarrays) - exposure 2 (4 microarrays) 4h : exposure 1 (2 microarrays) - exposure 2 (2 microarrays) 24h: exposure 1 (4 microarrays) - exposure 2 (6 microarrays) - exposure 3 (4 microarrays)
Project description:au10-04_phytoremediation; impact of sucrose on the tolerance of phenanthrene Effect of phenanthrene and sucrose - We test 3 conditions plants non-treated (C or t0), plants treated with phenanthrene (P) and plants tread with phenanthrene and sucrose (S). The plants were grown on MS/2 media for 17 days and then transferred on the corresponding condition. We took a sample of 30 plants at different times (0, 30 min, 2h, 4h, 8h and 24h).
Project description:Purpose: The goal of this study is to profile genes expression in mice liver during liver regeneration process post 2/3 partial hepatectomy using RNA-seq. Methods: Mice aged 8~10 weeks were performed with either 70% partial hepatectomy(PH) or shamed operation. At 0.5h, 2h, 4h, 24h, 48h, 72h, 120h, 168h post PH and after shamed operation, mice (six at each time point) were killed and liver samples were harvested. Total RNA were prepared from the liver samples using TRIZOL reagent (Invitrogen), and the mRNA profiles were genenated by Illumina HiSeq 2000. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Results: Our study provides a realiable dataset covering the three phases of liver regeneration: primer phase(0.5h,2h,4h), proliferation phase (24h, 48h, 72h), and termination phase (120h,168h). Using this dataset, we discover some interesting facts undiscovered before, especially on the re-organization process after proliferation wave and the termination phase.
Project description:Embelin (15 uM) induced alterations in the gene expression profile was studied in A549 lung cancer cells during the initial stages of apoptosis (4h following treatment)
Project description:The purpose of this work was to identify the potential signatures of cobalt exposure using a toxicogenomic approach. A time course transcriptome analysis was performed on human type II epithelial cell line A549. Cells were exposed to cobalt at midlog phase ; a medium without FCS containing cobalt (CoCl2, Sigma) or not was added for 30mn, 2h, 4h or 24h. Total RNA was isolated using the Quiagen RNeasy miniprep kit, and then labelled using the FairPlay Microarray Labelling Kit (Stratagene). Amino reactive Cy3- and Cy5- dyes (CyTMDye Post-Labelling Reactive Dye Pack, Amersham) were further chemically coupled to test and control cDNA respectively. After hybridization, microarrays were scanned with GenePix 4000B (Axon Instrument Inc., Forster City, CA). Cy3 and Cy5 fluorescence intensities for the spots were quantified after local subtraction of background noise using Genepix Pro 4.0 software (Axon Instrument Inc.) Keywords: Time course
