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ABSTRACT: Influence of temperature variation on gene expression and cocoon production in Bombyx mori Linnaeus, 1758 (Lepidoptera: Bombycidae)

PROVIDER: PRJNA975906 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR24726985_1.fastq.gz	Fastqsanger.gz
	SRR24726985_2.fastq.gz	Fastqsanger.gz
	SRR24726986_1.fastq.gz	Fastqsanger.gz
	SRR24726986_2.fastq.gz	Fastqsanger.gz
	SRR24726987_1.fastq.gz	Fastqsanger.gz

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Project description:Bombyx mori cocoon has a multi-layer structure that provides optimal protection for silkworm pupa. Research on the mechanical properties of the multi-layer structure revealed structure-property relationships of the cocoon. Here, we investigated the protein components of the B. mori cocoon in terms of its multi-layer structure. Liquid chromatography-tandem mass spectrometry identified 286 proteins from the multiple cocoon layers. In addition to fibroins and sericins, we identified abundant protease inhibitors, seroins and proteins of unknown function. By comparing protein abundance across layers, we found that the outermost layer contained more sericin1 and protease inhibitors and the innermost layer had more seroin1. As many as 36 protease inhibitors were identified in cocoons, indicating efficient inhibitory activities against a fungal protease. Thus, we propose that more abundant protease inhibitors in the outer cocoon layers may provide better protection for the cocoon. This study increases our understanding of the multi-layer mechanism of cocoons, and helps clarify the biological characteristics of cocoons.

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Changes in gene expressions associated with egg diapause in multivoltine silkworm Bombyx mori

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Project description:The European clam, Ruditapes decussatus (Linnaeus, 1758) is a bivalve mollusc of the family Veneridae native to the European Atlantic and Mediterranean coastal waters. Its production is exclusively based on natural recruitment, which is subject to high annual fluctuations due to adversely affected by pollution and other environmental factors. Microarray analyses have been performed in four gonadal maturation stages of two higly productive Portuguese wild populations (Ria Formosa in South and Ria de Aveiro in North) characterized by different responses to spawning induction.

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Dosage compensation in Bombyx mori is achieved by partial repression of both Z chromosomes in males

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SILKWORM WING SCALES LC-MSMS ANALYSIS RAW DATA

Project description:To identify the cuticular proteins in developing wing scales of Bombyx mori, we performed LC-MS/MS analysis of dissoliving developing wing scales from Bombyx mori

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