Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A human iPSC-derived multilineage liver organoid model containing Kupffer cells to study human sepsis-associated liver dysfunction [hiPSC-EMP-KC-pKC]

ABSTRACT: A human iPSC-derived multilineage liver organoid model containing Kupffer cells to study human sepsis-associated liver dysfunction [hiPSC-EMP-KC-pKC]

PROVIDER: PRJNA976631 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24745726.fastq.gz	Fastqsanger.gz
	SRR24745727.fastq.gz	Fastqsanger.gz
	SRR24745728.fastq.gz	Fastqsanger.gz
	SRR24745729.fastq.gz	Fastqsanger.gz
	SRR24745730.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

A human iPSC-derived multilineage liver organoid model containing Kupffer cells to study human sepsis-associated liver dysfunction [hiPSC-EMP-KC-pKC]

Project description:To incorporate Kupffer cells into hiPSC-LOs, we differentiated erythro-myeloid progenitors (EMPs) from hiPSCs. We compares the gene expression profiles of hiPSC-EMPs with cord-blood hematopoietic stem and progenitor cells (CB-HSPCs); and EMP-generated Kupffer cells (EMP-KC) with human primary Kupffer cells.

2024-03-01 | GSE233538 | GEO

Stutzerimonas stutzeri KC strain:KC

Project description:Stutzerimonas stutzeri KC strain:KC Genome sequencing

| PRJNA1044571 | ENA

Thermotoga sp. EMP

Project description:Thermotoga sp. EMP Genome sequencing and assembly

| PRJNA87045 | ENA

Combined effects of EMP and RF exposure on mouse hippocampus LC-MSMS

Project description:Recently, concerns about the combined effects of electromagnetic field (EMF) in daily living and occupational environment are rapidly growing. 4.9 GHz radiofrequency (RF) is commonly used in 5G telecommunication in daily life, EMP is common is occupational environment. Till now, the biological effects of combined exposure to EMP and 4.9 GHz RF have not been fully understood. We used LC-MSMS quantitative proteomics method to investigate the potential effects of EMP and RF field exposure in adult male mice model.

2023-05-10 | PXD038101 | Pride

Multilineage communication regulates human liver bud self-organization from pluripotency II

Project description:Multilineage communication regulates human liver bud self-organization from pluripotency II

| PRJNA380268 | ENA

Stutzerimonas stutzeri KC strain:KC

Project description:Pseudomonas stutzeri KC Genome sequencing and assembly

| PRJNA661097 | ENA

Citrobacter freundii strain:Cf-Emp

Project description:Citrobacter freundii strain:Cf-Emp Genome sequencing

| PRJNA899443 | ENA

Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids

Project description:Pluripotent stem cell (PSC)-derived organoids are emerging as novel human-based microphysiological models but display immature phenotypes with limited subsets of endothelial or stromal cells. Here we demonstrate that in vitro manipulation of gene regulatory networks (GRNs) in PSC-derived liver organoids selected either through computational analysis or targeted tissue design can advance tissue maturation in vitro. Through an unbiased comparison with the genetic signature of mature livers, we identify downregulated GRNs in fetal liver organoids compared to adult livers. We demonstrate that overexpression of PROX1 and ATF5, together with targeted CRISPR-based transcriptional activation of endogenous CYP3A4, drives maturation in vitro. Single cell analyses reveal hepatobiliary-, endothelial-, and stellate-like cell populations. The engineered organoids demonstrate enhanced vasculogenesis, capture native liver characteristics (e.g. FXR signaling, CYP3A4 activity), and exhibit therapeutic potential in mice. Collectively, our approach provides a genetically guided framework for engineering developmentally advanced multilineage tissues from hiPSCs.

2020-12-07 | GSE159491 | GEO

Single cell transcriptome analysis of primary murine EMP reveals complex erythroid, myeloid, and innate lymphoid lineage heterogeneity

Project description:Single cell transcriptome analysis of primary murine EMP reveals complex erythroid, myeloid, and innate lymphoid lineage heterogeneity

2021-07-05 | GSE143871 | GEO

Sphingomonas paucimobilis strain:EMP 5-4

Project description:Sphingomonas paucimobilis strain:EMP 5-4 Genome sequencing

| PRJNA1272276 | ENA