Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Skeletal Stem Cell Diversity

ABSTRACT: Human Skeletal Stem Cell Diversity

PROVIDER: PRJNA979132 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24809600_1.fastq.gz	Fastqsanger.gz
	SRR24809600_2.fastq.gz	Fastqsanger.gz
	SRR24809700_1.fastq.gz	Fastqsanger.gz
	SRR24809700_2.fastq.gz	Fastqsanger.gz
	SRR24809800_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 13172

Similar Datasets

Human Skeletal Development and Regenerative Capacity is Shaped by Skeletal Stem Cell Diversity

Project description:Human Skeletal Development and Regenerative Capacity is Shaped by Skeletal Stem Cell Diversity

| PRJNA979226 | ENA

Human Skeletal Stem Cell Diversity

Project description:We have used SmartSeq2 to sequence single phenotypic human skeletal stem cell (SSCs) and downstream lineage populations purified via FACS from fetal skeletal tissues and patient specimens. SSC populations were isolated based on their previously reported surface marker profiles. A human SSC (CD45-CD235a-CD31-TIE2-CD146-PDPN+CD73+CD164+), a human bone-cartilage-stroma-progenitor (BCSP; CD45-CD235a-CD31-TIE2-CD146+PDPN+) and an osteoprogenitor (OP; CD45-CD235a-CD31-TIE2-CD146+PDPN-) were investigated.

2025-02-25 | GSE233970 | GEO

Human Skeletal Development and Regenerative Capacity is Shaped by Skeletal Stem Cell Diversity

Project description:This SuperSeries is composed of the SubSeries listed below. The skeleton is one of the most structurally and compositionally diverse organ systems in the human body, depending on unique cellular dynamisms. Here, we integrate prospective isolation of human skeletal stem cells (hSSCs; CD45−CD235a−TIE2−CD31−CD146−PDPN+CD73+CD164+) from ten skeletal sites with functional assays and single-cell RNA sequencing (scRNA-seq) analysis to identify chondrogenic, osteogenic, stromal, and fibrogenic subtypes of hSSCs during development and their linkage to skeletal phenotypes. We map the distinct composition of hSSC subtypes across multiple skeletal sites and demonstrate their unique in vivo clonal dynamics. We find that age-related changes in bone formation and regeneration disorders stem from a pathological fibroblastic shift in the hSSC pool. Utilizing a Boolean algorithm, we uncover gene regulatory networks that dictate differences in the ability of hSSCs to generate specific skeletal tissues. Importantly, hSSC lineage dynamics are pharmacologically malleable, providing a new strategy to treat aberrant hSSC diversity central to aging and skeletal maladies.

2025-02-25 | GSE234040 | GEO

Skeletal Stem Cell Diversity in Mouse Long Bones

Project description:Skeletal Stem Cell Diversity in Mouse Long Bones

| PRJNA678366 | ENA

Skeletal Stem Cell Aging

Project description:Skeletal Stem Cell Aging

| PRJNA679960 | ENA

Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [CUT&Tag]

Project description:Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [CUT&Tag]

| PRJNA1215765 | ENA

Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [ATAC-seq]

Project description:Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [ATAC-seq]

| PRJNA1215763 | ENA

Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [bulkRNA-seq]

Project description:Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [bulkRNA-seq]

| PRJNA1215760 | ENA

Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [scRNA-seq]

Project description:Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [scRNA-seq]

| PRJNA1215762 | ENA

Modeling human limb skeletal development using human pluripotent stem cell-derived skeletal assembloids [CUT&Tag]

Project description:Despite recent advances in pluripotent stem cell-based approaches to induce skeletal cells, recapitulating human limb skeletal development in terms of structure and longitudinally oriented growth remains an unresolved challenge. Here, we report a method to differentiate human pluripotent stem cells into region-specific skeletal organoids harboring GDF5+PRG4+ interzone/articular chondrocyte　progenitors (IZ/ACPs) and SP7+ growth plate chondrocytes (GPCs) via PRRX1⁺ limb-bud mesenchymal cells. Comparative analysis demonstrated marked similarities of IZ/ACP and GPC organoids to the human embryonic limb, and graft fate and regenerative capacity in vivo were further characterized. We also mimicked the limb skeletal developmental process in a spatially structured manner by vertically positioning two IZ/ACP organoids at both ends of a GPC organoid to generate a human skeletal assembloid. Notably, this human skeletal assembloid recapitulated endochondral ossification with longitudinal skeletal growth upon transplantation. In summary, our study provides a novel research platform for human limb skeletal development and disease.

2025-01-26 | GSE288046 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data