Ontology highlight
ABSTRACT:
PROVIDER: PRJNA979916 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR27941445_subreads.fastq.gz | Fastqsanger.gz | |||
| SRR27997493_subreads.fastq.gz | Fastqsanger.gz | |||
| SRR28456531_1.fastq.gz | Fastqsanger.gz | |||
| SRR28456531_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 8 |
Similar Datasets
Project description:Between 2000 and 2015, mortality due to Alzheimer’s disease (AD) increased by 123%. No drugs have yet been approved to stop or slow the progression of AD. A delay of five years in the expression of AD would reduce the incidence rate by half. Thus, it is critical to develop novel prevention strategies to delay the onset of this common disease.
As an ancillary study conducted within a precision-based randomized trial (R01CA149633; PI, Dai & Yu]"), the investigators reduced Ca:Mg ratios to 2.3 through 3-month personalized Mg supplementation among those who consumed high Ca:Mg ratio diet, but otherwise in good general health. The investigators test the hypothesis that actively reducing the Ca:Mg ratio among those aged >65 years who consume high Ca:Mg ratio diets improves cognitive function compared to the placebo arm. The investigators further conduct molecular epidemiologic studies to understand the molecular mechanisms.
| 2324458 | ecrin-mdr-crc
Project description:Background: African Americans (AA) have increased burdens of cardiovascular disease and cancer compared to Caucasian Americans (CA). This study addresses the possibility that genetic differences affecting the biology of the vascular endothelium could be a factor contributing to this health disparity. Methods: From self-identified, healthy, 20-29 year old AA (n=21) and CA (n=17), we established cultures of blood outgrowth endothelial cells (BOEC) and applied microarray profiling. BOEC have never been exposed to in vivo influences, and their gene expression reflects culture conditions (meticulously controlled) and donor genetics. Analysis used two distinct approaches. Significance Analysis of Microarray, a FDR-based test, identified significant differential expression of single genes. Gene Set Enrichment Analysis examined expression of pre-determined gene sets that survey each of nine biological systems relevant to endothelial cell biology. Results: At the highly stringent threshold of FDR=0, we identified 31 single genes that were differentially expressed, 4 higher and 27 lower in AA. “PSPH” exhibited the greatest fold-change (AA>CA), but this was entirely accounted for by a homolog (PSPHL) hidden within the PSPH probe set. Among other significantly different genes were: for AA>CA, SOS1, AMFR, FGFR3; and for AA<CA, ARVCF, BIN3, EIF4B. Many more (221 transcripts for 204 genes) were differentially expressed at the more customary, less stringent threshold of FDR<.05. Using the biological systems approach, we identified shear response biology as being significantly altered for AA versus CA. It detected an apparent tonic increase of expression (AA>CA) for 46/157 genes within that system. Conclusions: The most significant single gene changes detected for AA involved genes having substantial, known roles in endothelial biology. Biological systems analysis suggested that shear stress response, a critical regulator of endothelial function and vascular homeostasis, may be different between AA and CA. These results potentially have direct implications for the role of endothelial cells in both vascular disease (e.g., hypertension and stroke) and cancer (via angiogenesis). The present findings are consistent with our overarching hypothesis that genetic influences stemming from ancestral continent-of-origin could impact upon endothelial cell biology and thereby contribute to disparity of vascular-related disease burden amongst AA.
2010-07-03 | GSE22688 | GEO
Project description:Biology and Molecular Analysis of Human Hematopoiesis Genetics
| PRJNA89541 | ENA
Project description:Biology and Molecular Analysis of Human Hematopoiesis Genetics
| PRJNA157239 | ENA
Project description:Primary outcome(s): Clinical and molecular chraateristuc of interval colon cancer (patient information, index colonoscopy result, pathology result, TNM stagingm, molecular biology marke( BRAF, CIMP (CpG island methylation phenotype), MSI, KRAS ??
Study Design: Observational Study Model : Cohort, Time Perspective : Prospective, Enrollment : 50, Biospecimen Retention : Collect & Archive- Sample with DNA, Biospecimen Description : Biopsy specimen of cancer tissue
| 2512257 | ecrin-mdr-crc
Project description:To understand molecular mechanisms that are deregulated in hematopoietic stem progenitor cells from transgenic mice that express the constitutively active form of IKK2 protein in HSCs (CA/CA mice), we isolated Flt3 low Lin-Sca1+c-Kit+ (LSK) cells from control and CA/CA mice and performed microarray analysis using the Illumina's MouseWG-6 v2.0 Expression BeadChip platform.
2018-10-13 | GSE121195 | GEO
Project description:We evaluated the anti-leishmanial efficacy of different saturated medium-chain fatty acids (FAs, C8-C18) where FA containing C8 chain, caprylic acid (CA), was found to be most potent against Leishmania donovani, the causative agent for visceral leishmaniasis (VL). Different analogs of CA with C8 chain, but not higher, along with a carboxyl/ester group showed a similar anti-leishmanial effect. Ergosterol depletion, measured by HPLC, was the major cause of CA-mediated cell death. Molecular docking and Molecular dynamic simulation studies indicated the enzyme mevalonate kinase (MevK) of the ergosterol biosynthesis pathway as a possible target of CA. Enzyme assays with purified recombinant MevK and CA/ CA analogs confirmed the target with a competitive inhibition pattern. The inhibition constant of CA was ⁓53µM. Using Isothermal calorimetry and circular dichroism studies; strong binding interaction between MevK and CA/CA analogs was established. Point mutation with Y167F residue at MevK active site reduces the binding affinity of CA on MevK. Also, homologous over-expression of MevK in parasites resisted CA-mediated killing. Further, proteomics studies using LC-HRMS showed dose-dependent down-regulation of MevK in CA-treated cells. We established the mechanism of the antileishmanial effect of CA, a natural product, against VL where toxicity and drug resistance with currents chemotherapeutics demand an alternative. This is the first report on the identification of an enzymatic target with kinetic parameters & mechanistic insights against any organism for a natural medium-chain FA. CA and, even, its derivatives/analogs may be developed as an anti-parasitic formulation with higher efficacy and reduced toxicity.
2022-10-14 | PXD034560 | Pride
Project description:We examined 22 comatose patients resuscitated after CA and obtained peripheral blood from the patients at 48 hours after CA. To identify novel blood biomarkers, we aimed to measure neurological outcomes according to the Cerebral Performance Category (CPC) score at 6 months after CA and to determine blood transcriptome-based molecular signature of poor neurological outcome group.
2017-01-01 | GSE92696 | GEO