Project description:The assess the effect of AvrSr35 knock-out on host-pathogen interaction in the compatible host, we have performed time-course analysis of leaf transcriptomes obtained by infecting susceptible wheat cultivar Fielder with the wild type Puccinia graminis f.sp. tritici (Pgt) isolate 99KS76A-1 and its three mutants, M1, M4 and M7, that carry loss-of-function mutations in the AvrSr35 gene.
Project description:Lettuce is a widely consumed leafy vegetable, valued for its nutritional benefits and culinary versatility. Various breeding initiatives have yielded lettuce varieties such as romaine or cos lettuce (Lactuca sativa var. longifolia), characterized by pigmented germplasm and enhanced tolerance to elevated temperatures. Despite its significance, a notable need exists for targeted study investigations into its chemical composition. Thus, this study aims to define the volatile and non-volatile components of the red romaine Thurinus RZ lettuce cultivar using LC-MS and GC-MS analysis. For LC-MS analysis, a C18 column will be accompanied by gradient elution using (A) water and (B) acetonitrile, each containing 0.1% formic acid as the mobile phase. For GC-MS analysis, the HP-5 column will be accompanied by Helium as the carrier gas. The molecules determined will then be ionized, and fragments will be interpreted and annotated using the mass-to-charge (m/z) ratio, which will further be tabulated.
Project description:Transcriptional profiling of pear tree comparing a resistant/tolerant cultivar with a susceptible cultivar to the Stemphylium vesicarium fungus Rocha' pear is an economically important portuguese Pyrus communis L. cultivar very susceptible to the Stemphylium vesicarium pathogenic fungus, the brown spot agent, causing huge decrease on fruit quality and yield production. Field control of brown spot disease is based in systemic application of antifungal chemicals with high economic costs and dramatic consequences to public health and environmental pollution. Plant-pathogen interactions involve a series of events encompassing constitutive and induced plant defence responses whose dissection has been a research target for control many crop diseases. The biosynthesis of cell wall polymers and antifungal compounds appear to be an efficient physical and chemical barrier to infection.To understand the molecular responses behind defence mechanisms of resistant/tolerant and susceptible cultivars of Pyrus communis L. to the S. vesicarium fungus, cDNA microarray technology was used to identify the genes differentially expressed along a time course leaf inoculation between 'Rocha' pear cultivar (a high susceptible cultivar) and 'Ercolini' pear cultivar (a resistant/tolerant pear cultivar). This study aims to contribute with information on the molecular mechanisms involved in host-pathogen interactions responsible for pear tree brown spot disease and resistance to Stemphylium vesicarium.