Project description:The oxt6 mutant is an oxidative stress-tolerant Arabidopsis mutant that is deficient in a polyadenylation factor subunit. Expression analysis suggests that impaired poly(A) site choice is responsible for the stress-tolerant phenotype. We used microarrays to understand the link between the polyadenylation defect and stress tolerance. Keywords: mutant, wild-type, complemented line comparison
Project description:The oxt6 mutant is an oxidative stress-tolerant Arabidopsis mutant that is deficient in a polyadenylation factor subunit. Expression analysis suggests that impaired poly(A) site choice is responsible for the stress-tolerant phenotype. We used microarrays to understand the link between the polyadenylation defect and stress tolerance. Experiment Overall Design: Arabidopsis plants (the wild-type, oxt6 mutant, and two independent oxt6 transformants each carrying a gene encoding just the smaller of the two At1g30460-encoded proteins) were seeded and grown in soil. Plants were harvested for RNA isolation after 30 days; for this, six samples derived from 10-15 plants were prepared for each line. Ten samples were selected for study â three wild-type, three oxt6, and two from each of the complemented lines. Analysis of independent complemented plants minimizes the possible effects that may arise due to T-DNA insertion.
Project description:Use 3ʹ region extraction and deep sequencing (3'READS) and bioinformatics techniques to profile alternative polyadenylation and gene regulation in plant Arabidopsis thaliana exposed to light and darkness
Project description:We performed transcriptome and ribosome immunoprecipitation studies of transgenic Arabidopsis (Arabidopsis thaliana) Columbia seeds expressing a His6FLAG-tagged version of the ribosomal large subunit protein L18B (35S:HF-RPL18B)
Project description:we investigated the effect of exposing 4 successive generations of Arabidopsis thaliana to severe heatwave conditions. We show that heat-primed offspring has increased survival rates and seed production after exposure to heat wave conditions. Bisulfite sequencing allowed us to link this to altered DNA methylation, including a global hypomethylation of the mitochondrial DNA. mRNA sequencing showed that priming affected genes related to the mitochondrial energy system and oxidative stress responses.
