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Human monocytes exposed to SARS-CoV-2 display features of trained immunity producing high levels of CXCL10 upon restimulation

ABSTRACT: Human monocytes exposed to SARS-CoV-2 display features of trained immunity producing high levels of CXCL10 upon restimulation

PROVIDER: PRJNA984320 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24940331.fastq.gz	Fastqsanger.gz
	SRR24940332.fastq.gz	Fastqsanger.gz
	SRR24940333.fastq.gz	Fastqsanger.gz
	SRR24940334.fastq.gz	Fastqsanger.gz
	SRR24940335.fastq.gz	Fastqsanger.gz

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Similar Datasets

Human monocytes exposed to SARS-CoV-2 display features of trained immunity producing high levels of CXCL10 upon restimulation

Project description:The capacity of innate immune cells to build immunological memory after a first encounter with certain pathogens or vaccines that allow them to respond stronger to reinfection is referred to as “trained immunity”. Despite few studies highlighting an important role for trained immunity in protection during COVID-19 infection or vaccination, no study so far has shown whether SARS-CoV-2 can train innate immune cells to respond stronger to a second stimuli. The aim of this study was to investigate whether this virus can induce trained immunity in human monocytes. To this purpose we used monocytes from healthy donors and exposed them to inactivated (i) SARS -CoV-2 for 24 hours followed by a resting period of several days in medium only and restimulation on day 6. On day 6, to evaluate the effects of iSARS-CoV-2 training on monocyte gene expression, transcriptomic analysis of human monocytes, before or 3h after LPS stimulation, was done. Our data provide the transcriptional profile of untrained and trained monocytes with iSARS-CoV-2.

2023-12-20 | GSE235094 | GEO

Human Monocytes Exposed to SARS-CoV-2 Display Features of Innate Immune Memory Producing High Levels of CXCL10 upon Restimulation.

Project description:IntroductionA role for innate immune memory in protection during COVID-19 infection or vaccination has been recently reported. However, no study so far has shown whether the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can train innate immune cells. The aim of this study was to investigate whether this virus can induce trained immunity in human monocytes.MethodsMonocytes were exposed to inactivated SARS-CoV-2 (iSARS-CoV-2) for 24 h, followed by a resting period in the medium only and a secondary stimulation on day 6 after which the cytokine/chemokine and transcriptomic profiles were determined.ResultsCompared to untrained cells, the iSARS-CoV-2-trained monocytes secreted significantly higher levels of IL-6, TNF-α, CXCL10, CXCL9, and CXCL11 upon restimulation. Transcriptome analysis of iSARS-CoV-2-trained monocytes revealed increased expression of several inflammatory genes. As epigenetic and metabolic modifications are hallmarks of trained immunity, we analyzed the expression of genes related to these processes. Findings indicate that indeed SARS-CoV-2-trained monocytes show changes in the expression of genes involved in metabolic pathways including the tricarboxylic acid cycle, amino acid metabolism, and the expression of several epigenetic regulator genes. Using epigenetic inhibitors that block histone methyl and acetyltransferases, we observed that the capacity of monocytes to be trained by iSARS-CoV-2 was abolished.ConclusionOverall, our findings indicate that iSARS-CoV-2 can induce properties associated with trained immunity in human monocytes. These results contribute to the knowledge required for improving vaccination strategies to prevent infectious diseases.

| S-EPMC10718582 | biostudies-literature

oxLDL exposed trained monocytes

Project description:oxLDL exposed trained monocytes

| PRJNA504428 | ENA

RNA-seq of human PMA-differentiated cell line THP-1 trained with DCATH-2 peptide against untreated controls after a 3-day rest followed by stimulation with medium or lipopolysaccharide

Project description:Trained innate immunity has been shown for human macrophages, i.e. a primary stimulation with specific compounds such as Beta-glucans, BCG vaccine or oxidized LDL generates an innate memory capacity that leads to an amplified pro-inflammatory reaction to various microbial components when restimulated days later. In this study, phorbol ester differentiated THP-1 cells were used as a model for human macrophages. The aim of this experiment was to map the biological processes involved in DCATH-2 peptide trained THP-1 cells versus control cells without restimulation and after restimulation with lipopolysaccharides.

2022-04-19 | E-MTAB-11601 | biostudies-arrayexpress

scRNAseq of monocytes from Trained immunity experiments

Project description:scRNAseq of monocytes from in vitro Trained immunity experiments stimulated by β-glucan (BG), uric acid (UA), muramyl dipeptide (MDP), oxidized low-density lipoprotein (oxLDL), or RPMI-Control, and respective samples restimulated with Lipopolysaccharide (LPS).

2021-09-30 | E-MTAB-9702 | biostudies-arrayexpress

Epigenomes of oxLDL exposed trained monocytes

Project description:Epigenomes of oxLDL exposed trained monocytes

| PRJNA504432 | ENA

Transcriptomes of Hemin exposed trained monocytes

Project description:Transcriptomes of Hemin exposed trained monocytes

| PRJNA435107 | ENA

Transcriptomes of oxLDL exposed trained monocytes

Project description:Transcriptomes of oxLDL exposed trained monocytes

| PRJNA504431 | ENA

Epigenomes of Hemin exposed trained monocytes

Project description:Epigenomes of Hemin exposed trained monocytes

| PRJNA435439 | ENA

Transcriptional remodeling of primary human monocytes trained with interleukin-4 (IL4)

Project description:Trained immunity is a phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to certain microbial components, altering their responses to future exposures. This study profiled the transcriptome of human macrophages trained with IL4 and then re-exposed to lipopolysaccharide.

2023-04-06 | GSE185433 | GEO

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