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ABSTRACT: Unravelling the role of venom in predator-prey interactions using natural populations and transgenic approaches in Nematostella vectensis

PROVIDER: PRJNA984949 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24955640.fastq.gz	Fastqsanger.gz
	SRR24955641.fastq.gz	Fastqsanger.gz
	SRR24955642.fastq.gz	Fastqsanger.gz
	SRR24955643.fastq.gz	Fastqsanger.gz
	SRR24955644.fastq.gz	Fastqsanger.gz

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Venom tradeoff shapes interspecific interactions, physiology and reproduction

Project description:An animal’s ability to effectively capture prey and defend against predators is pivotal to its survival1. A key innovation in many predator-prey interactions is venom, which consists of many toxin proteins that shape its phenotype2. Its unusually direct relationship of gene-toxin-phenotype make it an appealing system for studies at the organismal level 3,4. In this work we use the sea anemone Nematostella vectensis as a model organism as it provides us with the opportunity to test for the first time how toxin-genotypes impact predator-prey interactions. Specifically, we compare a native-population5 and a transgenic line which both have significantly reduced levels of Nv1, the major toxin in adult Nematostella6, to animals with wildtype levels of Nv1. We demonstrate that the transgenic strain phenocopies native anemones lacking Nv1 as they both have impaired ability to defend themselves against grass shrimp, a native predator. Mummichog killifish, unexpectedly are attracted to Nematostella with wildtype-levels of Nv1, highlighting that Nv1 plays a complex role in shaping interspecific-interactions. Finally, we demonstrate an evolutionary tradeoff as the reduction of Nv1 levels causes faster growth and increased reproductive rates compared to Nematostella control lines. Overall, our results experimentally link organism’s venom to its physiology, reproduction and interspecific interactions.

2023-12-31 | PXD043484 | Pride

Venom tradeoff shapes interspecific interactions, physiology and reproduction

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Dynamics of venom composition across a complex life cycle

Project description:Nematostella vectensis is a venomous organism with a complex life cycle. We harness different approaches to study venom composition dynamics along its life cycle. Our methods include but not limited to transcriptomica and proteomics. Here we perform semi quantitative analysis of Nematostella proteom at 4 developmental stages to compare abundance of different toxins.

2018-02-14 | PXD008218 | Pride

Cnidarian cell type diversity revealed by whole-organism single-cell RNA-seq analysis

Project description:We performed single-cell transcriptome analysis (using MARS-seq) in the cnidarian Nematostella vectensis. Additionally we performed bulk ATAC-seq experiments on wild-type specimens, as well as ELAV::mOrange reporter transgenic lines (separating ELAV+ and ELAV- cell populations, representing neurons and non-neurons, respectively).

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Nematostella toxins expression under stress

Project description:Changes in Nematostella vectensis proteome expression were analyzed in 2 different Nematostella populations along the east coast of USA in different stress conditions vrs. normal growth temperature.

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Project description:Cnidarians, including corals, sea anemones, and jellyfish, possess specialized stinging cells called cnidocytes that function in prey capture and defense. These cells represent a striking evolutionary innovation and include distinct types such as venom injecting nematocytes and mechanically acting spirocytes. While their biomechanics and transcriptional regulation have been studied extensively, little is known about their epigenetic regulation. Here, we combined epigenetic profiling with RNA sequencing in the sea anemone Nematostella vectensis to explore regulatory programs underlying cnidocyte diversity. We identified cell type specific regulatory elements in promoter and enhancer regions and linked them to distinct gene expression programs. This analysis revealed fundamental differences between nematocytes and spirocytes and uncovered a previously unrecognized nematocyte population that expresses the nep3 toxin but lacks most other toxins. These findings highlight the complexity of cnidocyte regulation and suggest greater cellular diversity within this defining cnidarian cell type than previously appreciated.

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Project description:NvNcol3::mOrange2 is a stable transgenic line that labels cnidocytes(stinging cells) of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvNcol3::mOrange2 positive and negative cells were enriched by FACS.

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Project description:NvElav1::mOrange is a stable transgenic line that labels a large fraction of the nervous system of the sea anemone Nematostella vectensis (Nakanishi et al., Development 2012). Two week old primary polyps were dissociated and the NvElav1::mOrange positive cells were enriched by FACS.

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| PRJNA831625 | ENA