Project description:Perfluorobutanoic acid (PFBA) is a short-chain polyfluoroalkyl substance (PFAS) that is measurable in fish tissues and environmental matrices. Previous studies suggest that there are adverse effects on animal and human health following exposure to PFBA, however the mechanisms underlying the toxicity are not well characterized. This study measured biological responses (developmental, transcriptome, and behavioral responses) of zebrafish (wild-type AB strain, Danio rerio) following exposure to a range of PFBA concentrations (0.1-100 µl/L). To further elucidate putative mechanisms related to locomotor abnormalities by PFBA, RNA-seq was conducted. These data shed new mechanistic light onto the sublethal effects of lesser studied short chain perfluorinated chemicals.
Project description:To study the expression profiles of hexaploid wheat chromosome 3B genes during the life cycle of a wheat plant and establish a transcriptome atlas for this chromosome, deep transcriptome sequencing was conducted in duplicates in 15 wheat samples corresponding to five different organs (leaf, shoot, root, spike, and grain) at three developmental stages each. Strand-non-specific and strand-specific libraries were used to produce 2.52 billion paired-end reads (232 Gb) and 615.3 single-end reads (62 Gb), respectively.
