Project description:We established chromate transformed cell lines by chronic exposure of normal human bronchial epithelial BEAS-2B cells to low doses of hexavalent chromium followed by anchorage-independent growth. The gene expression profiles were analyzed in the established cell lines. The gene expression profiles from six chromate transformed cell lines were remarkably similar to each other yet differed significantly from that of either control cell line or normal Beas-2B cells. A total of 409 differentially expressed genes were identified in chromate transformed cells compared to control cells.
Project description:We established chromate transformed cell lines by chronic exposure of normal human bronchial epithelial BEAS-2B cells to low doses of hexavalent chromium followed by anchorage-independent growth. The gene expression profiles were analyzed in the established cell lines. The gene expression profiles from six chromate transformed cell lines were remarkably similar to each other yet differed significantly from that of either control cell line or normal Beas-2B cells. A total of 409 differentially expressed genes were identified in chromate transformed cells compared to control cells. We analyzed gene expression profiles from 10 cell lines ( six chromated transformed cells lines, three control cell lines, and parental BEAS-2B cells) using Affymetrix Human Gene 1.0 ST array. No techinical replicates were performed.
Project description:Transcriptional profiling of Arabidopsis thalina seedlings in response to high (140 M-BM-5M) and low (20 M-BM-5M) concentrations of chromate (K2CrO4). Low concentrations of chromate (e.g. 40 M-BM-5M) promoted primary root growth, while high concentrations (e.g. 140 M-BM-5M) repressed growth and increased formation of root hairs, lateral roots and adventitious roots. Three-condition experiment, high (140 M-BM-5M) and low (20 M-BM-5M) concentrations of chromate (K2CrO4) vs non-chromate added (Control). Four biological replicates, one replicate per array.
Project description:Transcriptional profiling of Arabidopsis thalina seedlings in response to high (140 µM) and low (20 µM) concentrations of chromate (K2CrO4). Low concentrations of chromate (e.g. 40 µM) promoted primary root growth, while high concentrations (e.g. 140 µM) repressed growth and increased formation of root hairs, lateral roots and adventitious roots.
Project description:Remote lung injury after acute kidney inkury (AKI) is a common issue in humans and is lethal especially in critically-ill patients. We investigated kidney-lung interactions following AKI using single-cell RNA sequencing (scRNA-seq) of kidney and lung in a murine AKI model.
Project description:Helminth infection leads to lung remodelling. We used single cell ATAC sequencing (scRNA-seq) to analyze the changes in lung cells following exposure to N. brasiliensis at day 28 and day 45 post helminth infection
Project description:Helminth infection leads to lung remodelling. We used single cell RNA sequencing (scRNA-seq) to analyze the changes in lung cells following exposure to N. brasiliensis followed by subsequent exposure to SARS-COV2
