Uncovering the hidden threat: single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer
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ABSTRACT:
PROVIDER: PRJNA986451 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24990000.fastq.gz | Fastqsanger.gz | |||
| SRR24990001.fastq.gz | Fastqsanger.gz | |||
| SRR24990002.fastq.gz | Fastqsanger.gz | |||
| SRR24990003.fastq.gz | Fastqsanger.gz | |||
| SRR24990004.fastq.gz | Fastqsanger.gz |
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Project description:In order to identify factors involved in TMZ-resistance, we engineered different TMZ-resistant glioblastoma cell lines. Wildtype cells were treated twice a week in duplicate with a clinically relevant concentration of TMZ (33 µM) for multiple weeks, until two individual resistant subclones were generated. Gene expression profiling was performed to identify differentially expressed genes in the resistant cells compared to their wildtype cells.
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