Project description:SST expressing neurons are the most diverse cell type in the mouse cortex. We use scRNAseq on fixed tissue to validate the cell type differences described in live cells scRNAseq.
Project description:To investigate the effects of Dnmt1 Knockout in Sst expressing interneurons on celltype distribution in the embryonic brain, Sst-Cre/tdTomato/Dnmt1 loxp2 knockout mice and Sst-Cre/tdTomato control mice were analysed. 10 µm coronal sections of E16.5 brains were prepared for the Merscope platform using the commercially available Pan Neuro panel (Vizgen), covering 500 distinct transcripts.
Project description:Experiment was done as a part of multi-methodological description of Sst-neurons subtypes in mouse VTA, aiming to reveal their transcriptional profiles and neurotransmitter nature.