Project description:The signal 3-OH-PAME produced by the phc QS system in Ralstonia solanacearum is a conserved and important quorum sensing signal molecule in this genus. This signal participates in the activation of signal transduction by the PhcA regulatory factor, regulating the transcriptional control of 30% of the genes in Ralstonia solanacearum. Quorum quenching technology is a biocontrol strategy that mediates quorum sensing regulatory networks, and has achieved very significant results in the study of quenching AHL and DSF signals. Previous studies identified HS-18 as a biocontrol bacterium with quorum sensing-inhibiting effects on multiple quorum sensing signals. This study aimed to identify quorum quenching enzymes capable of degrading 3-OH PAME using HS-18, identifying six enzymes encoded by genes that exhibit degradation activity toward 3-OH PAME. Among these, gene 12725 demonstrated the most significant quorum quenching effect, while genes 24610 and 03720 also exhibited strong quorum quenching activity. Additionally, these quench enzymes can reduce the pathogenicity of Ralstonia solanacearum on tomatoes, demonstrating promising application prospects.
Project description:This study evaluated the biocontrol efficacy, growth-promoting potential, and molecular mechanisms of the actinomycete strain FJSM-07, identified via 16S rDNA sequencing as Streptomyces murinus.
2026-07-14 | GSE338066 | GEO
Project description:Genome-wide Sequencing Analysis and Biocontrol Potential Evaluation of the Biocontrol Bacterium Lysinibacillus fusiformis KBD-5
Project description:Bdellovibrio is a Gram-negative bacterium that preys upon other Gram-negative bacteria, including many pathogens, and as such has potential as a biocontrol agent. Little is known of the molecular and genetic control of Bdellovibrio’s attack upon its prey and of the nature of the HI phenotype. Here, we apply microarray technology to monitor changes of gene expression during the initial stages of prey infection to determine which predatory genes are important in this stage and to gain insight into possible regulatory mechanisms controlling the predation process. Comparison to gene expression during HI growth reveals a “predatosome” of genes specifically upregulated during predation and implicates some of those important in HI growth.
Project description:Gene BbSwi6 playes an essential role in asexual differentiation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal development was analyzed by using high throughput sequencing (RNA-Seq).
Project description:Bdellovibrio is a Gram-negative bacterium that preys upon other Gram-negative bacteria, including many pathogens, and as such has potential as a biocontrol agent. Little is known of the molecular and genetic control of Bdellovibrioâ??s attack upon its prey and of the nature of the HI phenotype. Here, we apply microarray technology to monitor changes of gene expression during the initial stages of prey infection to determine which predatory genes are important in this stage and to gain insight into possible regulatory mechanisms controlling the predation process. Comparison to gene expression during HI growth reveals a â??predatosomeâ?? of genes specifically upregulated during predation and implicates some of those important in HI growth. 3 replicates of attack phase cells and 3 replicates of Host-Independent grown cells were analysed on individual arrays.
