Project description:The goal of this study was to gain a better understanding of the genetic background of gonadal maturation of the European eel and to use gene expression profiles to identify predictive markers for broodstock selection that can be measured in blood samples. To find leads for maturation markers we performed a pilot deep-sequencing transcriptome analysis of ovarian tissue derived from a yellow eel, a prepubertal silver eel and a post-spawning matured eel. Among the best leads were two key players in steroidogenesis, namely pP450c17 and liver receptor homolog-1.
Project description:The goal of this study was to gain a better understanding of the genetic background of gonadal maturation of the European eel and to use gene expression profiles to identify predictive markers for broodstock selection that can be measured in blood samples. To find leads for maturation markers we performed a pilot deep-sequencing transcriptome analysis of ovarian tissue derived from a yellow eel, a prepubertal silver eel and a post-spawning matured eel. Among the best leads were two key players in steroidogenesis, namely pP450c17 and liver receptor homolog-1. Pilot deep-sequencing transcriptome analysis of ovary from a yellow, a prepubertal silver and a post-spawning matured eel
Project description:The aim of this study was to use a microarray approach to screen the major osmoregulatory tissues of the eel for changes in gene expression as fish acclimate to the SW environment. The other species (few clones) were used to validate the specificity of arrays hybridizations : Of the 6144 clones spotted, 96 contained control cDNAs comprising either known eel genes implicated in osmoregulation and previously studied in our laboratory or coding regions of genes sequenced from other species (ie. Aspergillus nidulans, Arabidopsis thaliana, Pleuronectes flesus, Carcharhinus leucas, Scyliorhinus canicula, Squalus acanthias and Salmo salar). The labelled cDNA probes derived from the eel RNAs hybridised only with the spotted cDNAs from the eel clones indicating the stringency and probe specificities of the hybridisation conditions were appropriate.
