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Effect of knockdown of KAT7 on gene expression in HCT116 cell line.

ABSTRACT: Effect of knockdown of KAT7 on gene expression in HCT116 cell line.

PROVIDER: PRJNA992108 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25178513_1.fastq.gz	Fastqsanger.gz
	SRR25178513_2.fastq.gz	Fastqsanger.gz
	SRR25178514_1.fastq.gz	Fastqsanger.gz
	SRR25178514_2.fastq.gz	Fastqsanger.gz

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Effect of knockdown of KAT7 on gene expression in HCT116 cell line.

Project description:Accurate centriole formation is critical for cell replication. However, the holistic transcriptional regulatory mechanisms involved in this process remain elusive.We report here that KAT7 histone acetyltransferase is a key regulatory molecule that regulates centriole replication. Knockdown of KAT7 leads to reduced levels of histone H3K14 acetylation and reduced enrichment of H3K14ac in the promoter region of centriole formation-related genes, resulting in blocked centriole formation. Our data provide a new insight for the role of histone modifying factors in centriole replication-related pathways.

2024-10-13 | GSE236701 | GEO

KAT7 modification in response to DNA damage LC-MS/MS analysis

Project description:To identify the acylation modification site of KAT7 in response to DNA damage, we treated HCT116 cells with etoposide, extracted proteins and purified KAT7 protein with KAT-specific antibodies for mass spectrometry analysis.This mass spectrometry identified the level of acylation modification of KAT7 in response to DNA damage in HCT116 cell line.

2024-12-12 | PXD043707 | Pride

KAT7 interaction in response to DNA damage LC-MS/MS analysis

Project description:To investigate the proteins bound to KAT7 in response to DNA damage, we harvested and extracted proteins after treating HCT116 with etoposide. The KAT7 protein and its binding protein were immunoprecipitated using KAT7-specific antibodies and analyzed by mass spectrometry. The mass spectra provided clues to study the function of KAT7 during DNA damage stress.

2024-12-12 | PXD043664 | Pride

Transcriptome profiling of HCT116 cells and HCT116 cells with knockdown of SETDB1 by RNA-sequencing

Project description:HCT116 is a colorectal cancer cell line. SETDB1 is an epigenetic modification factor responsible for catalyzing the histone modification H3K9me3. Transcription of SETDB1 gene is enriched in embryonic neural cells during vertebrate embryogenesis. SETDB1 is upregulated in cancer cells and promotes cancers. We found that knockdown of SETDB1 in HCT116 cells led to a neuronal-like differentiation phenotype.

2022-06-30 | GSE192372 | GEO

Neuroglobin (Ngb) Knockdown effect on glioblastoma cell line G422.

Project description:Neuroglobin (Ngb) Knockdown effect on glioblastoma cell line G422.

| PRJNA657020 | ENA

Effect of PLEK2 knockdown on the gene expression of HCT116 cells

Project description:To investigate the effect of PLEK2 in the regulation of HCT116 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of HCT116 cells.

2025-07-23 | GSE252372 | GEO

KAT7 promotes malignant phenotypes of colorectal cancer via MRAS-mediated activation of the MAPK/ERK pathway

Project description:Histone acetyltransferases (HATs) play a crucial role in transcriptional regulation by acetylating histones. Dysregulation of HAT activity is implicated in developmental disorders and cancer. In this study, we discovered an upregulated KAT7 signaling pathway in colorectal cancer (CRC) and its association with poor patient survival. Knockdown of KAT7 suppressed CRC cell viability, proliferation, migration, and invasion while promoting apoptosis. Conversely, KAT7 overexpression enhanced these cellular processes. In vivo experiments demonstrated that KAT7 knockdown inhibited CRC growth and lung metastasis. Mechanistically, KAT7 acetylated histone H3 at lysine 14 (H3K14) to enhance MRAS transcription, which activated the MAPK/ERK pathway and promoted tumorigenesis. Overexpression of MRAS or treatment with the ERK activator C6 Ceramide rescued the tumor inhibition caused by KAT7 silencing. The acetyltransferase activity of KAT7 is essential for CRC progression. Reexpression of KAT7, but not KAT7 acetyltransferase activity-deficient mutants, rescued MRAS expression, ERK phosphorylation, and CRC tumorigenesis in KAT7 knockdown CRC cells. Taken together, our results define the essential role of KAT7 in CRC tumorigenesis, rationalizing its potential as a therapeutic target for CRC treatment.

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Effect of GNAS mutation on the gene expression of HCT116 colon cancer cell line

Project description:To investigate the effect of activating GNAS mutation on gene expression of colorectal cancer cells, we utilized the GNAS R201C knockin cell line produced by Horizon Discovery Ltd. We compared the gene expression of parental and GNAS R201C/+ HCT116 cells with RNA-Seq, using four biological replicates.

2024-01-11 | GSE231943 | GEO

Ago2-bound ShortRnas in HCT116 cell line and Dicer_hypomorphic(DICER_Ex5 cells) HCT116 cell line

Project description:Ago2-bound ShortRnas in HCT116 cell line and Dicer_hypomorphic(DICER_Ex5 cells) HCT116 cell line

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Expression data from HCT116 cells following H19 knockdown

Project description:Knockdown of H19 leads to cell cycle arrest, reduced cell proliferation, and reduced cell migration in HCT116 cells. We used microarrays to detail the global programme of gene expression following H19 knockdown in HCT116 cells

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