Project description:Viral genomes are most vulnerable to cellular defenses at the start of the infection. A family of jumbo phages related to phage ΦKZ, which infects Pseudomonas aeruginosa, assembles a protein-based phage nucleus to protect replicating phage DNA, but how it is protected prior to phage nucleus assembly is unclear. We find that host proteins related to membrane and lipid biology interact with injected phage protein, clustering in an early phage infection (EPI) vesicle. The injected virion RNA polymerase (vRNAP) executes early gene expression until phage genome separation from the vRNAP and the EPI vesicle, moving into the nascent proteinaceous phage nucleus. Enzymes involved in DNA replication and CRISPR/restriction immune nucleases are excluded by the EPI vesicle. We propose that the EPI vesicle is rapidly constructed with injected phage proteins, phage DNA, host lipids, and host membrane proteins to enable genome protection, early transcription, localized translation, and to ensure faithful genome transfer to the proteinaceous nucleus.

2024-11-26 | PXD050275 | Pride

Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection

2024-11-26 | PXD049415 | Pride

Salmonella phage fSE4S isolate:KHS7

Project description:Salmonella phage fSE4S isolate:KHS7 Genome sequencing and assembly

| PRJNA291403 | ENA

Salmonella phage vB_SenP_SE4 isolate:vB_SenP_SE4

Project description:Salmonella phage vB_SenP_SE4 isolate:vB_SenP_SE4 Genome sequencing and assembly

| PRJNA1313466 | ENA

Viruses Caudovirales Siphoviridae Guernseyvirinae Jerseyvirus Salmonella phage vB_SenS-EnJE6

Project description:Viruses Caudovirales Siphoviridae Guernseyvirinae Jerseyvirus Salmonella phage vB_SenS-EnJE6 Genome sequencing

| PRJNA560145 | ENA