Single cell level characterization of injury-induced immune changes in 10-week-old and 95-week-old C57BL/6 knee joints
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ABSTRACT:
PROVIDER: PRJNA992491 | ENA |
REPOSITORIES: ENA
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Project description:Post-traumatic Osteoarthritis (PTOA) occurs in about half of individuals who sustained articular injuries including tibial plateau fracture, meniscus tear, or an anterior cruciate ligament (ACL) rupture. Damage-associated molecular pattern (DAMP) proteins are typically secreted from injured or dying cells of the synovial joint into the surrounding environment immediately following injury which in turn recruit leukocytes to mediate debris clean-up and repair. Prior bulk RNA-seq experiments exploring organismal age-related differences following ACL rupture found an increased expression of inflammatory-response related genes in 95-week-old mice relative to 10-week-old mice. Using single-cell RNA sequencing technology we temporally explored differences in immune cell infiltration in the synovial joint in 10-week-old and 95-week-old C57BL/6 mice.
2025-07-05 | GSE236843 | GEO
Project description:Single cell level characterization of immune cells in 10-week-old C57BL/6 knee joints and femur bone marrow
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Project description:Osteoarthritis (OA) of the hand is a common disease resulting in pain and impaired function. The pathogenesis of hand OA (HOA) is elusive and models to study it have not been described so far. Culture of chondrocytes is a model to study the development of cartilage degeneration, which is a hallmark of OA and well established in OA of the knee and hip. In the current study we investigated the feasibility human chondrocyte culture derived from proximal interphalangeal (PIP) finger joints of dissecting room cadavers. Index and middle fingers without signs of osteoarthritis were obtained from 30 cadavers using two different protocols. Hyaline cartilage from both articulating surfaces of the proximal interphalangeal (PIP) joint was harvested and digested in collagenase. Cultured chondrocytes were monitored for contamination, viability, and expression of chondrocyte specific genes. Chondrocytes derived from knee joints of the cadavers were cultured under identical conditions. Gene expression comparing chondrocytes from PIP and knee joints was carried out using Affymetrix GeneChip Human 2.0 ST arrays. The resulting differentially expressed genes were validated by real-time PCR and immunohistochemistry.Chondrocytes harvested up to 101 hours after death of the donors were viable. mRNA expression of collagen 2A1, aggrecan and Sox9 was significantly higher in chondrocytes as compared to cultured fibroblasts. Comparison of gene expression by chondrocytes from PIP and knee joints yielded 528 differentially expressed genes. Chondrocytes from the same joint region had a higher grade of similarity than chondrocytes of the same individual. These results were validated using real-time PCR and immunohistochemistry.We demonstrate for the first time a reliable method for culture of chondrocytes derived from PIP joints. PIP chondrocytes show a specific gene expression pattern and could be used as tool to study cartilage degeneration in HOA. Three samples of cultured chondrocytes from knee and proximal interphalangeal finger joints were compared. Gene expression of the four most differentially regulated genes was confirmed by real-time PCR in 10 independent samples.
2016-07-03 | E-GEOD-68038 | biostudies-arrayexpress
Project description:Osteoarthritis (OA) of the hand is a common disease resulting in pain and impaired function. The pathogenesis of hand OA (HOA) is elusive and models to study it have not been described so far. Culture of chondrocytes is a model to study the development of cartilage degeneration, which is a hallmark of OA and well established in OA of the knee and hip. In the current study we investigated the feasibility human chondrocyte culture derived from proximal interphalangeal (PIP) finger joints of dissecting room cadavers. Index and middle fingers without signs of osteoarthritis were obtained from 30 cadavers using two different protocols. Hyaline cartilage from both articulating surfaces of the proximal interphalangeal (PIP) joint was harvested and digested in collagenase. Cultured chondrocytes were monitored for contamination, viability, and expression of chondrocyte specific genes. Chondrocytes derived from knee joints of the cadavers were cultured under identical conditions. Gene expression comparing chondrocytes from PIP and knee joints was carried out using Affymetrix GeneChip Human 2.0 ST arrays. The resulting differentially expressed genes were validated by real-time PCR and immunohistochemistry.Chondrocytes harvested up to 101 hours after death of the donors were viable. mRNA expression of collagen 2A1, aggrecan and Sox9 was significantly higher in chondrocytes as compared to cultured fibroblasts. Comparison of gene expression by chondrocytes from PIP and knee joints yielded 528 differentially expressed genes. Chondrocytes from the same joint region had a higher grade of similarity than chondrocytes of the same individual. These results were validated using real-time PCR and immunohistochemistry.We demonstrate for the first time a reliable method for culture of chondrocytes derived from PIP joints. PIP chondrocytes show a specific gene expression pattern and could be used as tool to study cartilage degeneration in HOA.
2016-01-01 | GSE68038 | GEO
Project description:Injuries to the anterior cruciate ligament (ACL) often result in post-traumatic osteoarthritis (PTOA). PTOA accounts for ~12% of all osteoarthritis (OA) cases, yet the mechanisms contributing to OA after joint injury are not well understood. To better understand the molecular mechanisms behind PTOA development following ACL injury, we profiled ACL injury-induced gene expression changes in knee joints of three mouse strains with varying susceptibility to PTOA: STR/ort (highly susceptible), C57BL/6 (moderately susceptible) and super-healer MRL/MpJ (not susceptible) and identified genes differentially expressed between these strains at 0-day [before injury], 1-day, 1-week, and 2-weeks post-injury. This study highlights many new potential therapeutic targets and OA biomarkers.
2018-11-28 | GSE112641 | GEO
Project description:We report differences in gene expression between wildtype and Slc7a5 knockout CD4+ T cells obtained from mouse knee joints with antigen induced arthritis
2022-12-21 | GSE168750 | GEO
Project description:Trem2 is a single-pass transmembrane receptor of the immunoglobulin superfamily and is primarily expressed in myeloid cells. Cells from injured and uninjured knee joints of wildtype C57BL/6 mice and Trem2 KO mice were sequenced to determine if Trem2 plays a role in macrophage differentiation or function after joint injury.
2022-07-18 | GSE200842 | GEO
Project description:Synovial joints are structures where two bones meet and essential for mobility. For example, knee joints allow flexion and extension in a single plan for stable walking motions, whereas phalangeal joints together with skeletal muscles facilitate fine motor movements in hands. Joint is enclosed in a capsule and lined by a synovial membrane inside. Articular cartilage covering bone surfaces provide a wear-resistant structure that reduces friction during movement. Meniscus acts like a shock absorber to avoid direct grinding between bone surfaces. Cruciate ligaments reinforce the integrity of a joint by mounting two bones in place and limit the degree of joint movement. All these complex structures with different shapes are fit within the joint capsule for purpose. We established lineage maps of individual joint lineages for human and mouse to understand the progenitors, transcriptional regulators, signaling cues and extracellular matrix (ECM) involved in each differentiation trajectory. We asked whether LGR5+ joint progenitor cells are presence in early stage and decline with maturation as in our findings of mouse, and whether LGR5 and COL22A1 demarcate the chondrogenic and ligamentogenic lineages in human.
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Project description:Profiling the knee joints of mice with Type 1 diabetes mellitus
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Project description:Post-traumatic Osteoarthritis (PTOA) occurs in about half of individuals who sustained articular injuries including tibial plateau fracture, meniscus tear, or an anterior cruciate ligament (ACL) rupture. We utilize a noninvasive tibial compression injury (TCI) model that can closely replicate the anterior cruciate ligament rupture in patients, which reliably initiates PTOA development. In other studies we utilize single-cell RNA sequencing to temporally explore differences in immune cell infiltration following injury. Although extreme care is taken during immune cell isolation to prevent contamination from bone marrow, we also wanted to rule out the possibility of contamination from bone marrow immune cells in our knee joint samples. Therefore, we proceeded to compare the neutrophil populations from uninjured joint (joint) to bone marrow (marrow) to identify the similarities and differences between these populations.
2025-07-05 | GSE236844 | GEO