Project description:JHM80 strain was obtained by adaptive evolution of Methylomonas sp. DH-1 strain against 8.0 g/L of lactate. The tolerance against lactate of JHM80 was obtained by the overexpression of watR (weak acid tolerance regulator). In order to discover the target genes of WatR, FLAG epitope tag was added to WatR of JHM80 strain and ChIP-seq was performed.
Project description:The AlaRS mutant of Methylomonas DH-1 showed different lactate tolerance compared to the wild type. Proteomic analysis was conducted to find out what protein changes are related to this reason in the AlaRS mutant strain.
