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Synergistic Effect of Ultrasound and Wirelessly Powered Electrical Stimulation on Piezoelectric Membranes for Enhanced Osteogenesis by Immunomodulation of Macrophage Polarization

ABSTRACT: Synergistic Effect of Ultrasound and Wirelessly Powered Electrical Stimulation on Piezoelectric Membranes for Enhanced Osteogenesis by Immunomodulation of Macrophage Polarization

PROVIDER: PRJNA993117 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25222236_1.fastq.gz	Fastqsanger.gz
	SRR25222236_2.fastq.gz	Fastqsanger.gz
	SRR25222237_1.fastq.gz	Fastqsanger.gz
	SRR25222237_2.fastq.gz	Fastqsanger.gz
	SRR25222238_1.fastq.gz	Fastqsanger.gz

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Collagen-Enhanced Piezoelectric PLLA/ZnO Microfiber Barrier Membranes for Superior Bone Regeneration

Project description:Repairing large bone defects remains a significant clinical challenge. This study explores the development of collagen-enhanced piezoelectric microfiber barrier membranes (PLLA/ZnO@COL) for bone regeneration. The membranes' physicochemical properties, especially their piezoelectric characteristics, significantly influenced cellular behavior. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) confirmed the uniform dispersion of ZnO nanoparticles within the PLLA matrix, essential for optimal piezoelectric performance. The inclusion of collagen layers enhanced the electrical properties, cytocompatibility and osteogenic activity of the membranes. In vitro analyses, including bioinformatics, revealed that PLLA/ZnO@COL membranes upregulated key osteogenic pathways such as PI3K-AKT, MAPK, and Ras, thereby enhancing osteoblast proliferation, migration, and differentiation. Low-intensity pulsed ultrasound (LIPUS) stimulation further amplified these effects, indicating a synergistic role in enhancing osteoblast functions. In vivo studies using a rat mandibular bone defect model demonstrated that PLLA/ZnO@COL membranes, with the collagen layer facing the defect, significantly fostered new bone formation compared to control groups. Micro-CT analysis at 6 and 12weeks post-implantation showed higher bone mineral density, volume, and trabecular number in the PLLA/ZnO@COL group, indicating greater osteogenic activity. In conclusion, PLLA/ZnO@COL membranes, in conjunction with LIPUS, effectively enhance osteoblast functions and induce bone regeneration, showing potential as innovative barrier membranes in bone tissue engineering. The combination of piezoelectric properties, collagen enhancement, and LIPUS stimulation presents a novel approach to improving bone healing outcomes.

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Synergistic Effect of Ultrasound and Wirelessly Powered Electrical Stimulation on Piezoelectric Membranes for Enhanced Osteogenesis by Immunomodulation of Macrophage Polarization

Project description:Effect of Low-intensity pulsed ultrasound (LIPUS) assisted BT/MWCNTs/Col (BMC) membrane on gene expression during immune response of macrophages (Raw 264.7).

2023-07-31 | GSE236912 | GEO

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Project description:This study explored Rochelle salt-based biodegradable piezoelectric devices for applications in nerve regeneration and intestinal motility monitoring. Defective rat sciatic nerves were repaired using Rochelle salt-based biodegradable piezoelectric conduits, which were stimulated with transcutaneous ultrasound, leading to enhanced axon growth. High-throughput sequencing was employed to identify significantly altered genes.

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Collagen-Enhanced Piezoelectric PLLA/ZnO Microfiber Barrier Membranes for Superior Bone Regeneration

Project description:Collagen-Enhanced Piezoelectric PLLA/ZnO Microfiber Barrier Membranes for Superior Bone Regeneration

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Project description:Piezoelectric stimulation can promote neuronal differentiation of neural stem cells. Here we report a piezoelectric nanostickers that activate voltage-gated calcium channels (VGCC) on the surface of neural stem cells under ultrasound, inducing calcium ion influx.The influx of calcium ions leads to the upregulation of CaMKⅡ expression and phosphorylation, which initiates downstream signal transduction pathways in the process of neurogenesis. Phosphorylated CaMKⅡ (P-CaMKⅡ) enters the nucleus, where it facilities the phosphorylation of CREB. The phosphorylation of CREB enhances the expression of target gene, BDNF, ultimately leading to the completion of neurogenesis.

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Transcriptomic response of in vitro potato (Solanum tuberosum L.) to piezoelectric ultrasound

Project description:As part of a wider project to assess the impact of ultrasound on in vitro plant growth, this paper aimed to determine whether the application of piezoelectric ultrasound (PE-US) would induce changes to the transcriptome of in vitro potato (Solanum tuberosum L.). After exposing explants (single-node segments with a single leaf) to PE-US (35 kHz; 70 W) for 20 min, the effect of this stressor was determined at 0 h, 24 h, 48 h, 1 w and 4 w to assess the possible immediate and residual effects of PE-US on the potato transcriptome.

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Project description:Bone defect repair remains a major clinical challenge. This study presents a novel strategy using a 3D-printed piezoelectric hydrogel scaffold—composed of gelatin methacrylate (GelMA), hydroxyapatite (HA), and barium titanate (BTO)—for functional bone tissue engineering. The GelMA/HA/BTO (GelHABT) scaffold exhibited a well-defined porous structure, enhanced mechanical stability, and, crucially, reliable piezoelectric responsiveness. This key feature enables the material to convert external mechanical stimuli, such as low-intensity pulsed ultrasound (LIPUS), into endogenous electrical signals. In vitro, the scaffold promoted BMSCs adhesion, proliferation, and osteogenic differentiation, with performance significantly enhanced under LIPUS stimulation. Mechanistic insights revealed that the piezoelectric microenvironment remodeled the cellular miRNA expression profile, particularly up-regulating osteogenesis-related miR-29b-3p and activating the AMPK signaling pathway. Collectively, this ultrasound-responsive, gene-regulating scaffold represents a promising approach for treating bone defects by leveraging piezoelectricity to actively stimulate bone regeneration.

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Transcriptomic response of in vitro potato (Solanum tuberosum L.) to piezoelectric ultrasound

Project description:Transcriptomic response of in vitro potato (Solanum tuberosum L.) to piezoelectric ultrasound

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Metal-Free Perovskite Piezoelectric Nanogenerators for Human-Machine Interfaces and Self-Powered Electrical Stimulation Applications.

Project description:Single crystal metal-free halide perovskites have received great attention in recent years owing to their excellent piezoelectric and ferroelectric properties. However, the nanotoxicity and piezoelectricity within the nanoscale of such materials have yet been reported for the demonstration of practical applications. In this work, the observation of intrinsic piezoelectricity in metal-free perovskite (MDABCO-NH4 I3 ) films using piezoresponse force microscopy (PFM) is reported. A cytotoxicity test is also performed on MDABCO-NH4 I3 to evaluate its low-toxic nature. The as-synthesized MDABCO-NH4 I3 is further integrated into a piezoelectric nanogenerator (PENG). The MDABCO-NH4 I3 -based PENG (MN-PENG) exhibits optimal output voltage and current of 15.9 V and 54.5 nA, respectively. In addition, the MN-PENG can serve as a self-powered strain sensor for human-machine interface applications or be adopted in in vitro electrical stimulation devices. This work demonstrates a path of perovskite-based PENG with high performance, low toxicity, and multifunctionality for future advanced wearable sensors and portable therapeutic systems.

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Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes.

Project description:Electrical and/or electromechanical stimulation has been shown to play a significant role in regenerating various functionalities in soft tissues, such as tendons, muscles, and nerves. In this work, we investigate the piezoelectric polymer polyvinylidene fluoride (PVDF) as a potential substrate for wireless neuronal differentiation. Piezoelectric PVDF enables generation of electrical charges on its surface upon acoustic stimulation, inducing neuritogenesis of PC12 cells. We demonstrate that the effect of pure piezoelectric stimulation on neurite generation in PC12 cells is comparable to the ones induced by neuronal growth factor (NGF). In inhibitor experiments, our results indicate that dynamic stimulation of PVDF by ultrasonic (US) waves activates calcium channels, thus inducing the generation of neurites via a cyclic adenosine monophosphate (cAMP)-dependent pathway. This mechanism is independent from the well-studied NGF induced mitogen-activated protein kinases/extracellular signal-regulated kinases (MAPK/ERK) pathway. The use of US, in combination with piezoelectric polymers, is advantageous since focused power transmission can occur deep into biological tissues, which holds great promise for the development of non-invasive neuroregenerative devices.

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