Project description:We carried out a comparative genomic analysis of 48 avian species to identify avian-specific highly conserved elements (ASHCEs). We performed genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) for three enhancer-associated histone modifications (H3K4me1, H3K27ac, H3K27me3), to investigate dynamic regulatory roles of ASHCEs in chicken development. We found that all three enhancer-associated histone marks are enriched in ASHCEs compared to the whole genome background.
Project description:The aim of the study was to assess the effects of low pathogenic avian A/turkey/Massachusetts/3740/1965 [H6N2] influenza virus on a novel chicken lung epithelial cell line CLEC213. CLEC213 cells were mock-infected (4 samples) or infected with H6N2 virus (4 samples) at a multiplicity of infection (MOI) 1. Total RNA was collected 24 hours post-infection. To reduce potential experimental biases, RNA samples were collected from 4 independent experiments.
Project description:Effect of Lactobacillus plantarum MB452 on the gene expression of the intestinal epithelial cell line Caco-2 using a reference design Experiment Overall Design: Effect of Lactobacillus plantarum MB452 on the gene expression of the intestinal epithelial cell line Caco-2 using a reference design
