Project description:Xylose-utilizing yeasts with tolerances to fermentation inhibitors (such as weak organic acids) and high temperature are needed for cost-effective simultaneous saccharification and co-fermentation (SSCF) of lignocellulosic materials. We constructed a novel xylose-assimilating Saccharomyces cerevisiae strain with improved fermentation performance under heat and acid co-stress using the genome shuffling technique. Two xylose-utilizing diploid yeasts with different genetic backgrounds were used as the parental strains for genome shuffling. The hybrid strain Hyb-8 showed significantly higher xylose fermentation ability than both parental strains (Sun049T-Z and Sun224T-K) under co-stress conditions of heat and acids. To screen for genes that might be important for fermentation under heat and acid co-stress, a transcriptomic analysis of hybrid strain Hyb-8 and its parental strains was performed.
Project description:The main objectives of this study were to expand our understanding of NSF1 gene function in industrial S. cerevisiae M2 strain during fermentation by finding the largest maximal clique of co-expressed genes (i.e. Interdependent Correlation Cluster), and to establish the impact of Nsf1p on genome-wide gene expression during the fermentation process with possible implications related to wine quality and S. cerevisiae adapation to stressful fermentation conditions The Affymetrix Yeast 2.0 microarrays were used to capture the global gene expression profile of M2 and M2 nsf1∆ grown under fermentation conditions in Riesling grape must at 18°C with no shaking at various time points. The analysis of this microarray dataset expanded our understanding of the mechanism of action and the roles of NSF1 under fermentation stress conditions.
Project description:Interventions: Genomic test CANCERPLEX-JP OncoGuide NCC oncopanel system FndationONe CDx genome profile GUARDANT360 MSI Analysis System BRACAnalysis
Primary outcome(s): Development of genome database
Study Design: Single arm Non-randomized
| 2643590 | ecrin-mdr-crc
Project description:Metagenomic characterization of microbial fermentation bed system and ectopic fermentation system
| PRJNA524932 | ENA
Project description:Parallel dark fermentation system
Project description:The main objectives of this study were to expand our understanding of NSF1 gene function in industrial S. cerevisiae M2 strain during fermentation by finding the largest maximal clique of co-expressed genes (i.e. Interdependent Correlation Cluster), and to establish the impact of Nsf1p on genome-wide gene expression during the fermentation process with possible implications related to wine quality and S. cerevisiae adapation to stressful fermentation conditions The Affymetrix Yeast 2.0 microarrays were used to capture the global gene expression profile of M2 and M2 nsf1M-bM-^HM-^F grown under fermentation conditions in Riesling grape must at 18M-BM-0C with no shaking at various time points. The analysis of this microarray dataset expanded our understanding of the mechanism of action and the roles of NSF1 under fermentation stress conditions. The overall experimental setup consisted of 2 stains (M2 and M2 nsf1M-bM-^HM-^F) and 3 sample time points (24h post-innoculation, 20% and 85% of total glucose fermented) .
Project description:In conditions of nitrogen limitation, Saccharomyces cerevisiae strains differ in their fermentation capacities, due to differences in their nitrogen requirements. A population of 133 individuals from the F2 segregant population from a cross between two strains with different nitrogen requirements for efficient fermentation has been analyzed for their fermentation capacities. Two groups of 15 strains were defined, one group of High and one of Low Nitrogen requirement. These two groups are compared in order to detect genomic regions involved in the differences of nitrogen requirement. We used a custom isothermal array that has been designed for the detection of SNP at 6317 position on RM11.1a genome sequence http://www.broadinstitute.org/annotation/genome/saccharomyces_cerevisiae.3/Home.html) and obtained from the comparison with the genome sequence of strain Saccharomyces P3-D5.
